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11945

Supelco

Benedict′s reagent

for the qualitative determination of sugars

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12164500
NACRES:
NA.21

Quality Level

technique(s)

photometry: suitable

impurities

cupric sulfate

application(s)

food and beverages
general analytical

InChI

1S/C6H8O7.CH2O3.Cu.2Na.H2O4S.H2O/c7-3(8)1-6(13,5(11)12)2-4(9)10;2-1(3)4;;;;1-5(2,3)4;/h13H,1-2H2,(H,7,8)(H,9,10)(H,11,12);(H2,2,3,4);;;;(H2,1,2,3,4);1H2/q;;+2;2*+1;;/p-4

InChI key

QFKWSRIUZIYLCK-UHFFFAOYSA-J

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General description

Benedict′s reagents comprise of copper sulphate, sodium carbonate which functions as a mild alkali, and sodium citrate. It is quite stable. On boiling this reagent with glucose, it gives a white precipitate of cuprous thiocyanate. It is primarily used for the estimation of glucose in urine.

Application

Benedict′s reagent may be used during TLC analysis of mesocotyl methanol extracts during a study to evaluate sorghum resistance associated with accumulation of undetected toxic host metabolites. It may also be also be used to synthesize an SEM image of the cubic shaped Cu2O with smooth surface.

Other Notes

Reagent used for the detection of reducing sugars and other reducing substances; detection limit: 0.01 % glucose in water

Pictograms

Exclamation markEnvironment

Signal Word

Warning

Hazard Statements

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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R.L. Shriner, et al.
The Systematic Identification of Organic Compounds (1980)
R L Nicholson et al.
Proceedings of the National Academy of Sciences of the United States of America, 84(16), 5520-5524 (1987-08-01)
Infection of the sorghum mesocotyl by Helminthosporium maydis (a nonpathogen) and Colletotrichum graminicola (a pathogen) resulted in the rapid accumulation of a pigment complex by two sorghum cultivars. The components of the complex were fungitoxic. The principal compounds have been
Textbook of Medical Biochemistry.
Ramakrishnan S
Science, 18-19 (2004)
[Caustic lesions caused by test tablets used in semiquantitative urinary sugar determination (Clinitest)].
I Schlatter-Lanz
Schweizerische Rundschau fur Medizin Praxis = Revue suisse de medecine Praxis, 74(50), 1402-1406 (1985-12-10)
Fatal accidental ingestion of Clinitest in adult.
H J O'Connor et al.
Journal of the Royal Society of Medicine, 77(11), 963-965 (1984-11-01)

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