10127655001

Glucose-6-Phosphate Dehydrogenase (G6P-DH)

grade I, from yeast

• Synonym(s): G6P-DH, Glucose-6-Phosephate Dehydrogenase
• Enzyme Commission number: 1.1.1.49 (BRENDA, IUBMB)
• UNSPSC Code: 12352204

Properties

• biological source: yeast
• Quality Level: 100
• form: suspension
• specific activity: ~350 units/mg protein (At 25 °C with glucose-6-P as the substrate.)
• packaging: pkg of 1 mL (5 mg/ml)
• manufacturer/tradename: Roche
• concentration: ≥0.1-1.0 % (w/w)
• technique(s): activity assay: suitable
• color: white to yellowish
• optimum pH: 9.2
• solubility: water: miscible
• suitability: suitable for UV spectrophotometry and general use
• NCBI accession no.: CAA93357
• UniProt accession no.: P11412
• application(s): life science and biopharma
• foreign activity: 6-PGDH <0.0100%; CK <0.00100%; GR <0.0100%; HK <0.0100%; PGI <0.00200%; PGluM <0.0100%
• shipped in: wet ice
• storage temp.: 2-8°C
• Gene Information: Saccharomyces cerevisiae ... ZWF1(855480)

Description

General description

Glucose-6-phosphate dehydrogenase (G6P DH) has an active dimeric form, whereas its monomeric and tetrameric forms are inactive. At low NADP⁺ and NADPH levels and under normal conditions, the enzyme exists as equilibrium of tetramers and dimers, whereas at high NADP⁺ and NADPH levels the equilibrium shifts to inactive tetramers.

Application

Component of cofactor recycling systems for NADPH.

Biochem/physiol Actions

Glucose-6-phosphate dehydrogenase (G6P-DH) is involved in the pentose phosphate pathway. It is responsible for catalysing the oxidation of glucose-6-phosphate (G6P) to gluconolactone-6-phosphate, with NADP⁺ simultaneously accepting hydrogen to form NADPH, thereby maintaining NADPH levels in cells.

Specifications

Contaminants: <0.001% CK, <0.01% GR, HK, 6-PGDH, and PGluM each, <0.002% PGI each, <0.002% PGI

Approximately 350U/mg at +25°C with glucose-6-phosphate as the substrate.

Physical form

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

Preparation Note

Activator: Mg²⁺ (5 to 10 mM)

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): does not flash
• Flash Point(C): does not flash