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Roche

Papain

from Carica papaya

Synonym(s):

cystein protease, papain

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About This Item

Enzyme Commission number:
UNSPSC Code:
12352204

biological source

papaya

Quality Level

form

suspension

specific activity

30 U/mg
~30 units/mg protein (At 25 °C with BAEE as the substrate.)

mol wt

23 kDa

packaging

pkg of 10 mL (100 mg)

manufacturer/tradename

Roche

concentration

10-100 mg/mL

optimum pH

6.0-7.0

shipped in

wet ice

General description

Papain is a cysteine protease of the papain family. It is a single chain protein. The molecular weight of papain is 23 kDa. Papain is composed of two structural domains and a cleft between them, which consists of the active site.

Application

Papain has been used in the digestion of various brain cells.
Use Papain for the complete proteolytic cleavage of proteins, limited hydrolysis of native immunoglobulins, and tissue dissociation (together with collagenase, esterase, and trypsin). The enzyme solubilizes integral membrane proteins and produces glycopeptides from purified proteoglycans. The addition of cysteine (approximately 0.5% [w/v]) is essential for enzyme activity.

Biochem/physiol Actions

Papain exhibits proteolytic activity against amide links, amino acid esters, peptides, proteins. It mainly cleaves peptide bonds containing amino acids such as lysine, arginine and residues succeeding phenylalanine. It can catalyze several reactions, such as hydrolysis, transferase action, specificity and acyl-enzyme intermediate.

Features and Benefits

Inhibitors: SH-blocking reagents, iodoacetic acid, iodoacetamide, TPCK, TLCK, leupeptin, a2-macroglobulin, E-64, PMSF, and antipain, Hg2+, and other heavy metals

Physical form

Suspension, crystalline, nonsterile

Preparation Note

Working concentration: 0.05 to 0.5 mg/ml

Storage and Stability

Store at 2 to 8 °C. (a decrease in activity of 20% may occur within 6 months)

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

does not flash

Flash Point(C)

does not flash


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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LIM Kinase, a Newly Identified Regulator of Presynaptic Remodeling by Rod Photoreceptors After Injury.
Wang W and Townes-Anderson E
Investigative Ophthalmology & Visual Science, 56(13), 7847-7858 (2015)
The structure of papain
Advances in Protein Chemistry, 25, 79-115 (1971)
Logan A Walker et al.
Journal of translational medicine, 18(1), 63-63 (2020-02-11)
Direct cDNA preamplification protocols developed for single-cell RNA-seq have enabled transcriptome profiling of precious clinical samples and rare cell populations without the need for sample pooling or RNA extraction. We term the use of single-cell chemistries for sequencing low numbers
John S. White and Dorothy C. White
Source Book of Enzymes null
Monica Tambalo et al.
Development (Cambridge, England), 147(6) (2020-02-26)
Segmentation of the vertebrate hindbrain leads to the formation of rhombomeres, each with a distinct anteroposterior identity. Specialised boundary cells form at segment borders that act as a source or regulator of neuronal differentiation. In zebrafish, there is spatial patterning

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