Skip to Content
Merck
All Photos(2)

Key Documents

MAB378

Sigma-Aldrich

Anti-MAP2A Antibody, AP20

ascites fluid, clone AP20, Chemicon®

Synonym(s):

Microtubule Associated Protein 2

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

ascites fluid

antibody product type

primary antibodies

clone

AP20, monoclonal

species reactivity

quail, bovine, human, mouse, amphibian, Xenopus, rat, avian

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... MAP2(4133)

Related Categories

General description

MAP-2 (microtubule associated protein-2) is on of several high molecular weight proteins that play an important role in brain microtubule assembly. In addition to its association with microtubules, MAP-2 associates with neurofilaments and actin filaments suggesting that it may guide interaction among microtubules, other cytoskeletal elements, and cytoplasmic organelles (Binder, 1984).

MAP-2 is a stringent marker for neurons. In addition, MAP-2 displays intracellular specificity. In the central nervous system, MAP-2 is confined to neuronal cell bodies and dendrites. There are exceptions, however, where some axons stain positive for small amounts of MAP-2 (Caceres, 1984; Binder, 1986). MAP-2 is uniformly distributed throughout the cell when first expressed in cultured neurons but becomes selectively localized as dendritic development proceeds (Caceres, 1986; Dotti, 1987).

Specificity

MAP2a & b. Does not recognize other variants.

Immunogen

Bovine MAP2

Application

Detect MAP2A using this Anti-MAP2A Antibody, AP20 validated for use in IH & WB.
Immunohistochemistry:
1:50-1:200. AP20 stains dendrites and neuron cells bodies but not neuronal processes and axons. A previous lot of this antibody was used in immunohistochemistry.

Western blot:
SDS-PAGE Profiles: In SDS-PAGE MAP-2 from adult rat migrates as a closely associated doublet having a molecular weight of approximately 300 kD. However, early in brain development (postnatal day 10 in rats), MAP-2 migrates as a single band that is identical to the lower molecular weight band of the adult MAP-2 doublet (MAP-AP20). Later in development (postnatal days 17-18), the mobility of MAP-2 changes to the adult doublet form. (In the spinal cord, conversion to the adult form occurs earlier).

Western blot: A 1:500 dilution was used in a previous lot.

Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Neuronal & Glial Markers

Neurofilament & Neuron Metabolism

Quality

Routinely evaluated by Western Blot on PC12 lysates.

Western Blot Analysis:
1:500 dilution of this lot detected MAP 2 on 10 μg of PC12 lysates.

Target description

~ 300 kDa

Linkage

Replaces: MAB3418

Physical form

Unpurified
Unpurified mouse monoclonal IgG1 ascites fluid in buffer containing 15 mM sodium azide

Storage and Stability

Stable for 1 year at -20ºC in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Analysis Note

Control
Brain tissue

Cultured neurons

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Changqing Xu et al.
Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology, 11(2), 316-331 (2016-03-20)
In the era of combined antiretroviral therapy (cART), human immunodeficiency virus type 1 (HIV-1) is now considered a chronic disease that specifically targets the brain and causes HIV-1-associated neurocognitive disorders (HAND). Endocannabinoids exhibit neuroprotective and anti-inflammatory properties in several central
SDF1 reduces interneuron leading process branching through dual regulation of actin and microtubules.
Lysko, DE; Putt, M; Golden, JA
The Journal of Neuroscience null
Morphine and gp120 toxic interactions in striatal neurons are dependent on HIV-1 strain.
Podhaizer, EM; Zou, S; Fitting, S; Samano, KL; El-Hage, N; Knapp, PE; Hauser, KF
Journal of Neuroimmune Pharmacology null
Olfactory deficits in Niemann-Pick type C1 (NPC1) disease.
Hovakimyan, M; Meyer, A; Lukas, J; Luo, J; Gudziol, V; Hummel, T; Rolfs, A; Wree, A; Witt, M
Testing null
Carolina González et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(7), 1823-1828 (2016-02-04)
The regulation of the axonal proteome is key to generate and maintain neural function. Fast and slow axoplasmic waves have been known for decades, but alternative mechanisms to control the abundance of axonal proteins based on local synthesis have also

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service