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Key Documents

MAB1281C3

Sigma-Aldrich

Anti-Nuclei Antibody, clone 235-1, Cy3 conjugate

clone 235-1, from mouse, CY3 conjugate

Synonym(s):

HuNu

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

CY3 conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

235-1, monoclonal

species reactivity

human

technique(s)

immunocytochemistry: suitable

isotype

IgG1

shipped in

wet ice

target post-translational modification

unmodified

General description

Cell replacement techniques such as stem-cell or fetal-cell therapies show great promise for many diseases. Cross-species grafting between human and animal cells or tissues requires the identification and localization of the donor material in the host environment. Antibodies to human-specific nuclei are useful cytological and histological markers for identifying human cells in xenograft models. MAB1281 stains nuclei of all human and primate cell types giving a diffuse nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. The antibody does not react with nuclei from mouse or rat.

Immunogen

Human nuclei

Application

Evaluated by Immunocytochemistry in human bone marrow mesenchymal stem cells (SCR108). Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected nuclei in human bone marrow mesenchymal stem cells (SCR108). Evaluated by Immunocytochemistry in H9 human embryonic stem cells. Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected nuclei in H9 human embryonic stem cells. Evaluated by Immunocytochemistry in mouse embryonic fibroblasts. Immunocytochemistry Analysis: A 1:50 dilution of this antibody did not detect nuclei in mouse embryonic fibroblasts.
Research Category
Stem Cell Research

Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation

Developmental Neuroscience

Mesenchymal Stem Cells

Neurodegenerative Diseases
The anti-Nuclei, clone 235-1, Cy3 conjugate will recognize human cell nuclei only & is usefull in xenograph transplantation studies to study stem cell migration.

Quality

Evaluated by Immunocytochemistry in human foreskin fibroblasts (SCC058). Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected nuclei in human foreskin fibroblasts (SCC058).

Physical form

Protein G Purified
Purified mouse monoclonal IgG1 conjugated to Cy3 in PBS with 0.1% sodium azide and 15mg/ml BSA.

Storage and Stability

Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.

Analysis Note

Control
Human foreskin fibroblasts (SCC058)

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Hyun Ju Lee et al.
Journal of immunology (Baltimore, Md. : 1950), 194(8), 3634-3645 (2015-03-15)
Exogenously administered mesenchymal stem/stromal cells (MSCs) suppress autoimmunity despite transient engraftment. However, the mechanism is unclear. In this study, we report a novel mechanism by which MSCs modulate the immune system by recruiting myeloid-derived suppressor cells in a mouse model
Min Joung Lee et al.
Oncotarget, 8(39), 66281-66292 (2017-10-17)
Mesenchymal stromal cells (MSCs) have therapeutic potential for various diseases because of their anti-inflammatory and immunosuppressive properties. However, the immunosuppressive microenvironment allows tumor cells to evade immune surveillance, whereas maintenance of inflammation is required for tumor development and progression. Hence
Liying Zhang et al.
Circulation. Heart failure, 8(1), 156-166 (2014-11-26)
Induced pluripotent stem cells (iPSCs) can be differentiated into potentially unlimited lineages of cell types for use in autologous cell therapy. However, the efficiency of the differentiation procedure and subsequent function of the iPSC-derived cells may be influenced by epigenetic
Eleana Manousiouthakis et al.
Journal of tissue engineering and regenerative medicine, 13(9), 1712-1723 (2019-07-07)
Bidirectional interactions between the human central nervous system and the gastrointestinal tract, via the enteric nervous system, are unmapped and central to many human conditions. There is a critical need to develop 3D human in vitro intestinal tissue models to
Ludovic Zimmerlin et al.
Methods in molecular biology (Clifton, N.J.), 2416, 133-156 (2021-12-07)
Tankyrase/PARP inhibitor-regulated naïve human pluripotent stem cells (TIRN-hPSC) represent a new class of human stem cells for regenerative medicine that can differentiate into multi-lineage progenitors with improved in vivo functionality. Chemical reversion of conventional, primed hPSC to a TIRN-hPSC state

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