ECM550
QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), colorimetric
The CHEMICON Cell Invasion Assay Kit uses a 24-well plate, with 8 um pores, which provides an efficient system for evaluating the invasion of tumor cells through a basement membrane model.
Synonym(s):
Basement membrane cell invasion assay
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About This Item
Quality Segment
species reactivity
vertebrates
manufacturer/tradename
Chemicon®, QCM
technique(s)
cell based assay: suitable
detection method
colorimetric
shipped in
wet ice
storage temp.
2-8°C
General description
Introduction
Invasion through the extracellular matrix (ECM) is an important step in tumor metastasis. Cancer cells initiate invasion by adhering to and spreading along the blood vessel wall. Proteolytic enzymes, such as MMP collagenases, dissolve tiny holes in the sheath-like covering (basement membrane) surrounding the blood vessels to allow cancer cells to invade.
The CHEMICON Cell Invasion Assay Kit provides an efficient system for evaluating the invasion of tumor cells through a basement membrane model. The kit utilizes ECMatrix, a reconstituted basement membrane matrix of proteins derived from the Engelbreth Holm-Swarm (EHS) mouse tumor. We examined the kit′s performance using human fibrosarcoma (HT-1080) and non-invasive fibroblasts (NIH3T3).
The CHEMICON Cell Invasion Assay Kit is ideal for evaluation of invasive tumor cells. Each CHEMICON Cell Invasion Assay Kit contains sufficient reagents for the evaluation of 12 samples.
The CHEMICON Cell Invasion Assay Kit is intended for research use only; not for diagnostic or therapeutic applications.
Test Principle:
The CHEMICON Cell Invasion Assay is performed in an Invasion Chamber, a 24-well tissue culture plate with 12 cell culture inserts. The inserts contain an 8 μm pore size polycarbonate membrane, over which a thin layer of ECMatrixTM is dried. The ECM layer occludes the membrane pores, blocking non-invasive cells from migrating through. Invasive cells, on the other hand, migrate through the ECM layer and cling to the bottom of the polycarbonate membrane.
Application:
The CHEMICON Cell Invasion Assay Kit is ideal for evaluation of invasive tumor cells. Each CHEMICON Cell Invasion Assay Kit contains sufficient reagents for the evaluation of 12 samples.
The CHEMICON Cell Invasion Assay Kit is intended for research use only; not for diagnostic or therapeutic applications.
Application
Cell Structure
Packaging
Preparation Note
Other Notes
Cell Stain: (Part No. 20294) One 10 mL bottle.
Cotton Swabs: (Part No. 10202) 24 each.
Forceps: (Part No. 10203) One each.
Legal Information
Disclaimer
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| description The CHEMICON Cell Invasion Assay Kit uses a 24-well plate, with 8 um pores, which provides an efficient system for evaluating the invasion of tumor cells through a basement membrane model. | description The CHEMICON Cell Invasion Assay Kit employs a 96-well plate, and provides an efficient system for evaluating the invasion of tumor cells through a basement membrane model. | description The Cell Invasion Assay Kit kuses a 24 well plate with 8 um pores, which is ideal for evaluation of invasive tumor cells. | description This QCM Endothelial Cell Invasion Assay provides an in vitro model to quickly screen factors that can regulate endothelial invasion. The assay is performed in an invasion chamber using a basement membrane protein coated on the porous insert. |
| technique(s) cell based assay: suitable | technique(s) cell based assay: suitable, activity assay: suitable | technique(s) cell based assay: suitable | technique(s) cell based assay: suitable |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
| detection method colorimetric | detection method fluorometric | detection method fluorometric | detection method colorimetric |
| species reactivity vertebrates | species reactivity human | species reactivity vertebrates | species reactivity - |
| manufacturer/tradename Chemicon®, QCM | manufacturer/tradename QCM, Chemicon® | manufacturer/tradename Chemicon®, QCM | manufacturer/tradename QCM, Chemicon® |
Storage Class
10-13 - German Storage Class 10 to 13
wgk
WGK 1
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