ABN91

Anti-NeuN Antibody

from chicken, purified by affinity chromatography

• Synonym(s): RNA binding protein fox-1 homolog 3, Fox-1 homolog C
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: polyclonal
• Species reactivity: mouse, rat
• Application: ICC, IHC, WB
• Citations: 138

Properties

• biological source: chicken
• Quality Segment: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: mouse, rat
• technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable
• isotype: IgY
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Rbfox3(52897) ; rat ... Rbfox3(287847)

Description

General description

RNA binding protein fox-1 homolog 3 (UniProt: Q8BIF2; also known as Fox-1 homolog C, Hexaribonucleotide-binding protein 3, Fox-3, Neuronal nuclei antigen, NeuN antigen) is encoded by the Rbfox3 (also known as D11Bwg0517e, Hrnbp3) gene (Gene ID: 52897) in murine species. The NeuN protein is localized in nuclei and perinuclear cytoplasm of most neurons in the mammalian central nervous system. It is widely expressed in brain, including in cerebral cortex, hippocampus, thalamus, caudate/putamen, cerebellum, as well as in the spinal cord. However, it is not detected in Cajal-Retzius cells in the neocortex, Purkinje cells, and photoreceptor cells. NeuN expression has been reported as early as embryonic day 9.5 (E9.5) and by E12.5 it can be found in the developing ventral horns. It is also detected in the developing dorsal horns and in the dorsal root ganglion. Its expression is enhanced by retinoic acid treatment. It can serve as a pre-mRNA alternative splicing regulator and regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay. Initial characterization of NeuN was derived from usage of a monoclonal antibody (A60), but later identified as RBFOX3. Subsequent studies have shown that anti-NeuN antibodies can identify most types of neurons in the whole nervous system with some rare exceptions. NeuN knockout mice display significantly reduced brain weight, impaired neurofilament expression and decreased white matter volume. They exhibit increased susceptibility to seizures and reduced anxiety-related behaviors compared with their wild-type littermates. Antibodies for NeuN predominantly associate with cell nuclei and, to a lesser extent, with the perinuclear cytoplasm. Two isoforms of the NeuN protein (46 and 48 kDa) are present in both locations but differ in their relative concentration in the nucleus and cytoplasm. (Ref.: Lin, YS et al. (2018). PLOS One. 13(2); e0192355; Mao, S., et al. (2016). Front. Neuroanat. 13;10:54).

Target molecular weight ~47 and 52 kDa observed; 40.61 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Immunogen

Epitope: N-terminus

GST-tagged recombinant protein corresponding to the N-terminus of mouse NeuN.

Application

Anti-Neun, chicken polyclonal antibody detects Neun (RNA binding protein fox-1 homolog). It targets an epitope within 97 amino acids from the N-terminal.

Tested Applications

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected NeuN in rat E18 cortical cells.

Immunohistochemistry (Paraffin) Analysis: A 1:3,000 dilution from a representative lot detected NeuN in mouse brain tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

Biochem/physiol Actions

Other homologies: Human (94% sequence homology).

This antibody recognizes the N-terminus of NeuN.

Physical form

Purified chicken polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Affinity purified

Preparation Note

Recommended storage: +2°C to +8°C.

Analysis Note

Evaluated by Western Blotting in mouse E16 brain tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected NeuN in mouse E16 brain tissue lysate.

Control
Mouse brain E16 tissue lysate

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable