ABC102

Anti-CCN1/CYR61 Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Protein CYR61, CCN family member 1, Cysteine-rich angiogenic inducer 61, Insulin-like growth factor-binding protein 10, IBP-10, IGF-binding protein 10, IGFBP-10, Protein GIG1
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IHC, WB
• Citations: 4

Properties

• biological source: rabbit
• Quality Level: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human, mouse, rat
• species reactivity (predicted by homology): chimpanzee (based on 100% sequence homology)
• technique(s): immunohistochemistry: suitable (paraffin), western blot: suitable

Description

General description

CCN1 (cysteine-rich 61 or CYR61), a ubiquitous angiogenic factor, is a member of the CNN family, which is also comprised of CTGF, WISP-1, WISP-2, and WISP-3. It is a multidomain protein, containing one each of CTCK, IGFBP, TSP type-1, and VWFC domains. CCN1 is associated with the extracellular matrix and functions as a ligand for integrin receptors. It is expressed in response to growth factor stimulation, and mediates various cellular processes, including cell adhesion, migration, proliferation, angiogenesis, extracellular-matrix remodeling, and placental development. Previous studies have indicated that it may also play a role in wound healing by influencing the expression of genes required for this process, such as TIMP1, PAI-1, VEGA-A, VEGA-C, MMP1, MMP3, and uPA. CCN1 has also been implicated in breast cancer.

~53 kDa observed. The calculated molecular weight of this protein is 42 kDa, but can be observed at higher molecular weights due to glycosylation. A degradation fragment may be observed at ~15 kDa in some lysates. (Rother et al., 2010)

Immunogen

Epitope: CTCK domain

KLH-conjugated linear peptide corresponding to the CTCK domain of human CCN1/CYR61.

Application

Detect CCN1/CYR61 using this Anti-CCN1/CYR61 Antibody validated for use in Western Blotting, IHC(P).

Immunohistochemistry Analysis: 1:200 dilution from a representative lot detected CCN1/CYR61 in human kidney tissue.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Biochem/physiol Actions

This antibody recognizes the CTCK domain of: CCN1/CYR61

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
NIH/3T3 cell lysate

Evaluated by Western Blot in NIH/3T3 cell lysate.

Western Blot Analysis: 2 µg/mL of this antibody detected CCN1/CYR61 in 10 µg of NIH/3T3 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable