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Key Documents

AB5847

Sigma-Aldrich

Anti-Glutamate Receptor 1 Antibody, phosphoSer 831

Chemicon®, from rabbit

Synonym(s):

GluR1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat

packaging

antibody small pack of 25 μL

manufacturer/tradename

Chemicon®

technique(s)

western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... GRIA1(2890)

Specificity

Recognizes Glutamate Receptor 1 [GluR1], phosphoSer831. The antibody recognizes a protein of ~100 kDa corresponding to GluR1 phosphorylated at Ser831 in rat hippocampal homogenate. Immunolabeling of the GluR1 protein is blocked by the immunogen peptide but not by the corresponding non-phosphopeptide. The immunogen sequence is 100% homologous in human and mouse.

Immunogen

Epitope: phosphoSer 831
Synthetic peptide corresponding to amino acids surrounding the phosphoSer831 of rat GluR1.

Application

Detect Glutamate Receptor 1 using this Anti-Glutamate Receptor 1 Antibody, phosphoSer 831 validated for use in WB.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Western blot and dot blots: 1:500-1:1000. The working dilution should be based on the tissue and the expected phosphorylation state.

Optimal working dilutions must be determined by the end user.

Target description

100 kDa

Linkage

Replaces: 05-823

Physical form

Affinity Purified immunoglobulin. Liquid in 10 mM HEPES, pH 7.5, with 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
ImmunoAffinity Purified

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Hippocampal neurons, rat brain tissue lysates or mouse brain tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Mónica Fernández-Monreal et al.
Journal of cell science, 129(14), 2793-2803 (2016-06-04)
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