AB3786

Anti-SFTPC Antibody

CHEMICON^®, rabbit polyclonal

• Synonym(s): proSP-C
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• Clone: polyclonal
• Species reactivity: mouse, rat
• Application: IHC (p), WB
• Citations: 373

Properties

• Product Name: Anti-Prosurfactant Protein C (proSP-C) Antibody, serum, Chemicon^®
• biological source: rabbit
• Quality Segment: 100
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: mouse, rat
• manufacturer/tradename: Chemicon^®
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
• NCBI accession no.: NM_003018.3
• UniProt accession no.: P11686
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... SFTPC(6440), SP5(389058)

Description

General description

20 kDa observed. 21 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Pulmonary surfactant is a complex mixture of phospholipids and proteins secreted from airway epithelial cells (type II cells) in alveoli which reduces the surface tension at the alveolar air liquid interface, providing alveolar stability necessary for normal ventilation. Four distinct proteins isolated from pulmonary surfactant are termed surfactant proteins A, B, C and D. SP A (28 - 36 kDa) and SP D (43 kDa) are collagenous carbohydrate binding proteins, whereas SP B (8 - 9 kDa) and SP C (4 kDa) are non collagenous hydrophobic proteins. SP C is a fragment of a much larger precursor protein of 21 kDa. The precursor contains an exceedingly hydrophobic region of 34 amino acids that comprises most of the mature SP C, and a unique poly-valine domain.

Immunogen

GST-tagged recombinant protein corresponding to the N-terminal region of human Prosurfactant Protein C (proSP-C).

Application

• Western Blotting Analysis: A 1:500 dilution from a representative lot detected Prosurfactant Protein C (proSP-C) in mouse lung tissue lysate and GST-fusion Prosurfactant Protein C fragment.
• ELISA Analysis: A 1:50,000 dilution from a representative lot detected GST-fusion Prosurfactant Protein C fragment.
• Immunohistochemistry (Paraffin) Analysis: A 1:2,000 dilution from a representative lot detected Prosurfactant Protein C (proSP-C) in mouse lung tissue sections.
• Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Research Category
Metabolism

Research Sub Category
Ion & Transport Channels

This rabbit polyclonal Anti-Prosurfactant Protein C (proSP-C), Cat. No. AB3786 is tested for use in Western Blotting, Immunohistochemistry (Paraffin) and ELISA, for the detection of Prosurfactant Protein C (proSP-C).

Biochem/physiol Actions

Expected to cross-react with human.

Reacts strongly with proSP-C from human and mouse. Immunoprecipitates the 21 kDa proprotein as well as processing intermediates of M.W. 26 kDa, 21 kDa, 16 kDa and 14 kDa. Preincubation of the antiserum with the fusion protein results in ablation of immunostaining. On human tissue immunostains alveolar Type II cells in normal lung. The antibody also recognizes the aberrant form of proSP-C (6-12 kDa peptide) found in BAL and in alveolar proteinosis material on sections of lung samples from human patients with hereditary SP-B deficiency. On mouse tissue immunostains alveolar Type II cells in normal lung.

Physical form

Rabbit polyclonal antibody in serum with 0.05% sodium azide.

Unpurified

Preparation Note

Stable at -20ºC in undiluted aliquots for up to twelve months after date of receipt. Avoid repeated freeze/thaw cycles.

Analysis Note

Evaluated by Western Blotting in rat lung tissue lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected Prosurfactant Protein C (proSP-C) in rat lung tissue lysate.

Control
Mouse lung epithelial cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: 07-647

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable