69671
Thrombin, Restriction Grade
Synonym(s):
Thrombin, Restriction Grade
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About This Item
Recommended Products
biological source
human plasma
Quality Level
form
liquid
manufacturer/tradename
Novagen®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
technique(s)
protein extraction: suitable
suitability
suitable for additive or modifier in the separation of proteins or peptides
application(s)
life science and biopharma
shipped in
wet ice
storage temp.
−20°C
General description
Restriction Grade Thrombin is a highly purified preparation qualified to specifically cleave target proteins produced with appropriate vectors. This preparation is functionally tested for activity with fusion proteins and is free of detectable contaminating proteases. Prepared from human plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV. Thrombin is supplied as a solution in 50% glycerol and includes 10X Thrombin Cleavage Buffer and a Cleavage Control Protein.
Components
•50 UThrombin
•1 ml10X Thrombin Cleavage Buffer
•2 ml1X Thrombin Dilution/Storage Buffer
•10 µgCleavage Control Protein
•1 ml10X Thrombin Cleavage Buffer
•2 ml1X Thrombin Dilution/Storage Buffer
•10 µgCleavage Control Protein
Warning
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
Unit Definition
One unit is defined as the amount of enzyme needed to cleave 1 mg of fusion protein in 16 hours at 20°C in a 200 µl reaction containing 20 mM Tris-HCl pH 8.4, 150 mM NaCl, 2.5 mM CaCl₂, 50 µg fusion protein.
Preparation Note
Prepared from human plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Certificates of Analysis (COA)
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