69670-M
His•Bind® Resin
Synonym(s):
uncharged IDA agarose beads
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About This Item
UNSPSC Code:
41106500
NACRES:
NA.56
Recommended Products
form
liquid
manufacturer/tradename
Novagen®
storage condition
do not freeze
application(s)
life science and biopharma
storage temp.
2-8°C
General description
His•Bind Resin is an uncharged IDA agarose resin. The binding capacity is approximately 8 mg/ml bed volume. His•Bind Resin is well suited to small to medium scale purifications using either gravity flow columns or batch methods.
Application
His•Bind® Resin have been used: in purification of His•Tag® fusion proteins by metal chelation chromatography, for purification of the bacterially produced OCRL1WT/VAR phosphatase domain to study heterogeneity in Lowe Syndromein protein purification to study anaerobic phloroglucinol degradation by Clostridium scatologenes for isolation of His6-tagged proteins for studying the role of human repressor activator protein 1 (RAP1) and glial fibrillary acidic protein (GFAPɛ)proteins in increasing γ-secretase activity in a yeast model system.
Biochem/physiol Actions
His•Bind® resin helps in the purification of proteins containing the His-tagged sequence by the process of metal chelation chromatography. It utilizes the charge buffer provided in the His•Bind® Buffer Kit, that binds the His-tagged sequence effectively with Ni2+ or Co2+ cations that are immobilized onto the His•Bind® resin. The target protein can be easily recovered by eluting with imidazole. The His•Bind® Resin is designed to be regenerated and reused multiple times, making it a cost-effective solution. Its versatility allows for protein purification under gentle, non-denaturing conditions, or in the presence of either guanidine or urea, depending on the specific requirements of the purification process.
Features and Benefits
- Reusable many times
- Compatible with His•Bind® Buffer Kit
- Compatible with 1 mM THP
Warning
Toxicity: Standard Handling (A)
Legal Information
HIS-BIND is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Flam. Liq. 3
Storage Class Code
3 - Flammable liquids
WGK
WGK 1
Flash Point(F)
86.0 °F
Flash Point(C)
30 °C
Certificates of Analysis (COA)
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Find documentation for the products that you have recently purchased in the Document Library.
The human RAP1 and GFAP? proteins increase ?-secretase activity in a yeast model system
Swanson MJ, et al.
G3: Genes, Genomes, Genetics, 13(8) (2023)
Heterogeneity in Lowe Syndrome: Mutations Affecting the Phosphatase Domain of OCRL1 Differ in Impact on Enzymatic Activity and Severity of Cellular Phenotypes
J Lee J, et al.
Biomolecules, 13(4), 615-615 (2023)
Anaerobic phloroglucinol degradation by Clostridium scatologenes
Zhou Y, et al.
Environmental Microbiology, 14(4) (2023)
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