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α-Secretase Substrate II, Fluorogenic

Name: α-Secretase Substrate II, Fluorogenic
Brand: MM

An internally quenched fluorogenic peptide substrate containing the α-secretase cleavage site of β-Amyloid Precursor Protein (APP).

Synonym(s):

α-Secretase Substrate II, Fluorogenic, Ac-RE(EDANS)-VHHQKLVF-K(DABCYL)-R-OH

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About This Item

Empirical Formula (Hill Notation):

C₁₀₀H₁₄₄N₃₀O₂₀S

Molecular Weight:

2118.47

NACRES:

NA.77

UNSPSC Code:

12352200

Assay:

≥95% (HPLC)

Form:

solid

Storage condition:

OK to freeze, desiccated (hygroscopic), protect from light

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Properties

Quality Segment

100

assay

≥95% (HPLC)

form

solid

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze, desiccated (hygroscopic), protect from light

color

red

solubility

DMSO: 5 mg/mL

fluorescence

λ_ex ~340 nm, λ_em ~490 nm

shipped in

ambient

storage temp.

−20°C

Description

General description

An internally quenched fluorogenic peptide substrate that contains the α-secretase cleavage site of β-amyloid precursor protein (APP). Shown to be an effective substrate for yapsin-2, a glycosyl-phosphatidylinositol-linked aspartyl protease found in yeast (V_max/K_m = 0.031 min^-1). The cleavage has been shown to occur at the Lys-Leu bond. Excitation max: ~340 nm; emission max: ~490 nm.

Biochem/physiol Actions

Cell permeable: no

Primary Target
Substrate containing the α-secretase cleavage site of β-Amyloid Precursor Protein (APP)

Product does not compete with ATP.

Reversible: no

Packaging

Packaged under inert gas

Other Notes

Ac-Arg-Glu(EDANS)-Val-His-His-Gln-Lys-Leu-Val-Phe-Lys(DABCYL)-Arg-OH

Komano, H., et al. 1999. J. Biol. Chem. 274, 24431.
Komano, H., et al. 1998. J. Biol. Chem. 273, 31648.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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1 of 1

This Item	565758	565781	565764
Sigma-Aldrich 565767 α-Secretase Substrate II, Fluorogenic	Sigma-Aldrich 565758 β-Secretase Substrate IV, Fluorogenic	Sigma-Aldrich 565781 β-Secretase Substrate VI, Fluorogenic - Calbiochem	Sigma-Aldrich 565764 γ-Secretase Substrate, Fluorogenic
description An internally quenched fluorogenic peptide substrate containing the α-secretase cleavage site of β-Amyloid Precursor Protein (APP).	description A highly sensitive fluorescence resonance energy transfer (FRET) peptide substrate, based on the EVNLDAEF sequence derived from the β-secretase site of the Swedish mutation of Amyloid Precursor Protein (APP).	description -	description An internally quenched fluorogenic peptide substrate containing the C-terminal β-APP (amyloid β-peptide precursor protein) amino acid sequence that is cleaved by γ-secretase.
assay ≥95% (HPLC)	assay ≥95% (HPLC)	assay -	assay ≥95% (HPLC)
form solid	form lyophilized solid	form -	form lyophilized
manufacturer/tradename Calbiochem^®	manufacturer/tradename Calbiochem^®	manufacturer/tradename -	manufacturer/tradename Calbiochem^®
Quality Level 100	Quality Level 100	Quality Level 100	Quality Level 100
storage temp. −20°C	storage temp. −20°C	storage temp. -	storage temp. −20°C
solubility DMSO: 5 mg/mL	solubility DMSO: 5 mg/mL	solubility -	solubility 5% acetic acid: soluble, DMSO: 5 mg/mL

Storage Class

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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