53702

Pronase^® Protease, Streptomyces griseus

• Synonym(s): Pronase^® powder
• CAS Number: 9036-06-0
• UNSPSC Code: 12352202
• NACRES: NA.54
• MDL number: MFCD00132092
• eCl@ss: 32160410
• Specific activity: ≥45,000 proteolytic units/g dry wt

Properties

• description: RTECS - UK9595000
• Quality Segment: 100
• form: lyophilized
• specific activity: ≥45,000 proteolytic units/g dry wt
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, desiccated (hygroscopic)
• solubility: water: 10 mg/mL
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

A non-specific protease liquefies mucins and digests proteins to free amino acids. Can be used to isolate living chondrocytes. Most active at neutral pH, but it is stable over a wide pH and temperature range. Has an optimal pH of 7.5. Note: 1 KU = 1000 units.

Note: 1 KU = 1000 units.

Physical form

Lyophilized from a solution containing calcium acetate as a stabilizer.

Preparation Note

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for 24 h at 4°C.

Other Notes

Narahashi, Y., et al. 1968. J. Biochem. 64, 427.

One unit is defined as the amount of enzyme that will liberate a digestion product equivalent to 25 µg of tyrosine per min at 40°C, pH 7.5.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

PRONASE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Harmful (C)

Safety Information

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H315,H319,H334,H335
• pcodes: P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 11 - Combustible Solids
• wgk: WGK 2