48-611MAG
MILLIPLEX® Akt/mTOR Phosphoprotein Magnetic Bead 11-Plex Kit - Cell Signaling Multiplex Assay
Synonym(s):
Akt/mTOR Signaling immunoassay, Luminex® Cell Signaling Assay, Millipore Cell Signaling Assay, Signal Transduction Assay
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.47
Recommended Products
species reactivity
human
Quality Level
manufacturer/tradename
Milliplex®
technique(s)
multiplexing: suitable
detection method
fluorometric (Luminex® xMAP®)
storage temp.
2-8°C
General description
The Akt signaling pathway, one of the most often dysregulated signaling pathways in cancer, plays an important role in mediating a very broad range of cellular processes such as growth and development, cell cycle, energy homeostasis, and cell survival. Akt, a serine/threonine kinase that phosphorylates over 100 protein substrates, mediating cell survival and proliferation signals, is often itself hyperphosphorylated in various tumor types. Although the Akt gene itself is not known to be mutated in cancers, many of the upstream regulators of Akt, including IR, IRS, PI3K and PTEN, are oncogenes and tumor suppressors. Downstream of Akt, the mammalian Target Of Rapamycin (mTOR) complex is a key regulator of growth and metabolism. As nearly all of the players in the Akt/mTOR signaling pathway are coordinately regulated by phosphorylation, understanding the role of this pathway in normal physiological processes and in diseases such as cancer and diabetes requires the ability to simultaneously measure phosphorylation status of multiple protein targets.
Specificity
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Application
Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate samples. Compare Multiplexing results to those of Western blotting.
An overnight (4°C) incubation is recommended for best results.
This assay requires 25 μL diluted cell lysate per well.
This kit must be run using Assay Buffer 2 (provided).
The suggested working range of protein concentration for the assay is 15 to 150 ng of total protein/well (25 μL/well at 600 to 6,000 ng/mL).
Analytes available:
An overnight (4°C) incubation is recommended for best results.
This assay requires 25 μL diluted cell lysate per well.
This kit must be run using Assay Buffer 2 (provided).
The suggested working range of protein concentration for the assay is 15 to 150 ng of total protein/well (25 μL/well at 600 to 6,000 ng/mL).
Analytes available:
- Akt (Ser473)
- GSK3α (Ser21)
- GSK3β (Ser9)
- IGF1R (Tyr1135/Tyr1136)
- IR (Tyr1162/Tyr1163)
- IRS1 (Ser636)
- mTOR (Ser2448)
- p70S6K (Thr412)
- PTEN (Ser380)
- RPS6 (Ser235/Ser236)
- TSC2 (Ser939)
Packaging
96-well plate
Storage and Stability
Recommended storage for kit components is 2 - 8°C.
Legal Information
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2
Storage Class Code
10 - Combustible liquids
Certificates of Analysis (COA)
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