1.52027

Chromolith^® NH2 (monolithic) HPLC Columns

L × I.D. 50 mm x 4.6 mm HPLC Column

• Synonym(s): Chromolith^® SpeedROD NH2 50-4.6 HPLC column
• UNSPSC Code: 41115700
• NACRES: SB.52

Properties

• product name: Chromolith^® NH2, L × I.D. 50 mm x 4.6 mm HPLC column
• material: PEEK column
• Quality Level: 100
• Agency: suitable for USP L8
• product line: Chromolith^®
• feature: endcapped: no
• packaging: 1 ea of
• extent of labeling: 5% Carbon loading
• parameter: 200 bar pressure; 45 °C max. temp.
• technique(s): HPLC: suitable; LC/MS: suitable; mass spectrometry (MS): suitable (Low - Medium)
• column L × I.D.: 5 cm × 4.6 mm
• matrix: monolithic silica
• matrix active group: NH₂ bonding phase
• pore size: 13 nm mesoporosity; 2 μm macroporosity
• operating pH range: 2-7.5
• separation technique: hydrophilic interaction (HILIC); normal phase; reversed phase
• storage temp.: no temp limit

Description

General description

Chromolith^® columns are made of monolithic silica with a characteristic bimodal pore structure.

Chromolith^® NH₂ columns have macropores with 2 μm diameter, giving a column efficiency exceeding 80,000 plates/meter. The mesopores are 13 nm (130 Å). Chromolith^® NH₂ columns fall under the USP L8 Classification. The column efficiency is comparable to a 5 μm particle size fully porous particulate column. Chromolith^® NH₂ columns are suitable for LC-MS applications.
The column dimension of 50 mm length and 4.6 mm I.D. is the best choice for fast separations on any HPLC System.

The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity

The recommended Guard-columns are:
Chromolith^® NH₂ 5-4.6 guard cartridges (3 pieces) [1.52030] with the Chromolith^® Guard Cartridge Holder 5 mm x 4.6 mm [1.52032]
The Chromolith^® column coupler can be used to significantly increase the separation efficiency of Chromolith^® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467]

Learn more:
Brochure: Race through your Separations on Any System. Chromolith^® Monolithic Silica HPLC Columns

All Supelco HPLC Columns, including monolithic Chromolith^® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

Analysis Note

Pressure: ≤ 10 bar
Performance (2-nitroanisol)
Tailing (USP): 0.85 - 1.30 %
Theoretical Plates (N/m) (Cytosine): ≥ 80000
Capacity factor (Cytosine): ≥ 1.1

Legal Information

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany