07-2192

Anti-phospho-TBK1 (Ser172) Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Serine/threonine-protein kinase TBK1, NF-kappa-B-activating kinase, T2K, TANK-binding kinase 1
• eCl@ss: 32160702
• UNSPSC Code: 12352203
• NACRES: NA.41
• Conjugate: unconjugated
• Clone: polyclonal
• Application: WB
• Citations: -

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: mouse, human
• species reactivity (predicted by homology): rat (based on 100% sequence homology)
• technique(s): western blot: suitable
• NCBI accession no.: NP_037386
• UniProt accession no.: Q9UHD2
• shipped in: wet ice
• target post-translational modification: phosphorylation (pSer172)
• Gene Information: human ... TBK1(29110)

Description

General description

TBK, also known as IκB kinase (IKK)-related kinase NAK, belong to the serine/threonine IKK kinase family and is thought to play a role in NF-kappa-B (NF-κB) activation. The NF-κB complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NF-κB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NF-κB activation in response to certain growth factors. For example, the protein can form a complex with the IKB protein TANK and TRAF2 and release the NF-κB inhibition caused by TANK.

~90 kDa observed

Immunogen

KLH-conjugated linear peptide corresponding to human TBK1 phosphorylated at Ser172.

Application

This phospho-TBK1 antibody is validated for use in WB for the detection of the phospho-TBK1 protein.

Biochem/physiol Actions

This antibody recognizes TBK1 phosphorylated at Ser172.

Analysis Note

Control
EGF treated and untreated HEK293 cell lysates

Evaluated by Western Blot in EGF treated and untreated HEK293 cell lysates.

Western Blot Analysis: 1:1,000 dilution of this antibody detected TBK1 on 10 µg of EGF treated and untreated HEK293 cell lysates.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable