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Key Documents

L020000

Sigma-Aldrich

TCA Deblock

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About This Item

UNSPSC Code:
41116004
NACRES:
NA.21

type

for DNA synthesis
for DNA synthesis

Quality Level

product line

Proligo Reagents

form

liquid

storage condition

dry at room temperature

concentration

28-32 g/L (TCA content, titration)

technique(s)

DNA synthesis: suitable

impurities

≤200 ppm water content (Karl Fischer)

color

clear
clear

bp

39.8—40.0 °C/1,013 hPa

storage temp.

room temp

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Preparation Note

Trichloroacetic acid / Dichloromethane 3/100 (v/v)

Other Notes

Deblock solution containing trichloroacetic acid (TCA) in dichloromethane removes the dimethoxytrityl (DMT) protecting group from the 5′hydroxyl moiety of nucleotides already incorporated into the growing nucleic acid, prior to the addition of the next phosphoramidite. Removal of the DMT allows the unprotected 5′hydroxyl moiety to react with a new phosphoramidite in a subsequent extension reaction.

Signal Word

Danger

Hazard Classifications

Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Central nervous system, Respiratory system

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 2

Flash Point(F)

212.0 °F - closed cup

Flash Point(C)

100 °C - closed cup


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