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SAB4200658

Sigma-Aldrich

Monoclonal Anti-EPS8 antibody produced in mouse

clone EPS8-2, purified from hybridoma cell culture

Synonym(s):

DFNB102, epidermal growth factor receptor pathway substrate 8

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

EPS8-2

form

buffered aqueous solution

mol wt

antigen ~90 kDa

species reactivity

human

concentration

~1 mg/mL

technique(s)

flow cytometry: 10-20 μg/test using using A549 cells.
immunoblotting: 2.5-5 μg/mL using using whole extracts of HepG2 cells.
immunofluorescence: 10-20 μg/mL using using whole extracts of A549 cells.
immunoprecipitation (IP): suitable

isotype

IgG2a

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... EPS8(2059)

Related Categories

General description

Monoclonal Anti- EPS8 (mouse IgG2a isotype) is derived from the hybridoma EPS8-2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a sequence at the N-terminal region of human EPS8 (GeneID: 2059), conjugated to Key loop hemocyanin (KLH). Epidermal growth factor receptor kinase substrate 8 (EPS8) is also known as DFNB102 (autosomal recessive deafness-102). EPS8 is also found to be an actin filament barbed end capping protein.

Immunogen

synthetic peptide corresponding to a sequence at the N-terminal region of human EPS8

Application

Monoclonal Anti-EPS8 antibody produced in mouse has been used in:

  • immunoblotting
  • immunoprecipitation
  • immunofluorescence
  • flow cytometry

Biochem/physiol Actions

Epidermal growth factor receptor kinase substrate 8 (EPS8) serves as a substrate for the kinase activity of epidermal growth factor receptor (EGFR). It is essential for cell motility and regulation of actin filaments growth. EPS8 scaffolds together with Abl interactor 1 (Abi1) and SOS Ras/Rac guanine nucleotide exchange factor 1 (Sos1) to a trimeric complex which is required for activation of the small guanosine triphosphatase (GTPase) Rac. On the other hand, EPS8 form complexes with related to the N terminus of tre oncogene (RN-tre), a Rab5 GTPase activating protein, to inhibit EGFR internalization. EPS8 is involved in the regulation of processes such as axonal filopodia growth, stereocilia length, dendritic cell migration, cancer cell migration and invasion. It is reported to be expressed at elevated levels in a range of human malignancies. This protein is also involved in spine morphogenesis and plasticity and may characterize forms of intellectual disabilities. For instance, reduced EPS8 levels were reported in brains of autism patients.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A novel actin barbed-end-capping activity in EPS-8 regulates apical morphogenesis in intestinal cells of Caenorhabditis elegans
Croce A, et al.
Nature Cell Biology, 6(12), 1173-1173 (2004)
Elisabetta Menna et al.
The EMBO journal, 32(12), 1730-1744 (2013-05-21)
Actin-based remodelling underlies spine structural changes occurring during synaptic plasticity, the process that constantly reshapes the circuitry of the adult brain in response to external stimuli, leading to learning and memory formation. A positive correlation exists between spine shape and
Eps8 in the midst of GTPases
Di Fiore PP and Scita G
The International Journal of Biochemistry & Cell Biology, 34(10), 1178-1183 (2002)
Wael M Abdel-Rahman et al.
World journal of gastroenterology, 18(29), 3896-3903 (2012-08-10)
To analyze the epidermal growth factor receptor pathway substrate 8 (EPS8) expression status and role in colorectal carcinogenesis given that EPS8 has a conserved actin barbed-end capping function that is required for proper maturation in intestinal cells. We studied 8

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