S9415

Streptavidin−Maleimide from Streptomyces avidinii

• Synonym(s): Streptavidin Conjugate
• MDL number: MFCD00274720
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• extent of labeling: 4-10 mol maleimide per mol Streptavidin
• Quality Level: 200; 400
• suitability: suitable for direct conjugation to compounds containing free sulfhydryl groups
• storage temp.: −20°C

Description

General description

Streptavidin conjugated compounds may be used in avidin/strepavidin:biotin-based detection systems.

Streptavidin is a protein produced by the bacterium Streptomyces avidinii. It has four binding sites for biotin, as does avidin. Both have been used extensively as probes in immunochemical systems, conjugated to antibodies, enzymes or fluorochromes. However, avidin is highly basic (pI ~ 10.5), compared to streptavidin (pI ~ 5-6). Avidin has a slightly higher binding capacity for biotin than streptavidin does, but it has glycosylated side chains which give higher backgrounds in blotting techniques than does streptavidin, which is not a glycoprotein. Streptavidin is more resistant than avidin to dissociation into subunits by guanidinium chloride.

Application

Maleimide Activated Streptavidin is used to prepare streptavidin-protein conjugates with proteins, peptides and other molecules that contain a free sulfhydryl (-SH) group. Activated streptavidin presents an available maleimide group that reacts with sulfhydryl-containing molecules.

Physical form

Lyophilized powder containing sodium citrate.

Preparation Note

Streptavidin, from Streptomyces avidinii, activated with maleimidocaproic acid N-hydroxysuccinimide ester.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves