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RAB0326

Sigma-Aldrich

C-Peptide EIA Kit

for serum, plasma, culture supernatant and cell lysates

Synonym(s):

C-Peptide

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

human, mouse, rat

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
enzyme immunoassay: suitable

input

sample type plasma
sample type cell lysate
sample type culture supernatant(s)
sample type serum

assay range

inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 772 pg/mL
standard curve range: 0.1-1000 ng/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... INS(3630)

General description

The C-Peptide Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting C-Peptide peptide based on the principle of Competitive Enzyme Immunoassay. EIA-CPE-1 only detects C-peptide, not Insulin A or B chain.

Immunogen

C-Peptide , synthetic peptide

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

C-peptide inhibits endothelial dysfunction under high glucose conditions via inhibition of nuclear factor-κB (NF-κB) pathway. Experimental studies suggest that prolonged dosage of physiological concentration of C-peptide to type 1 diabetes patients minimizes diabetes-induced complications, such as glomerular hypertrophy and mesangial matrix expansion by interrupting transforming growth factor β1 (TGF-β1) and tumor necrosis factor α (TNFα) signaling pathway. C-peptide reduces diabetes-induced glomerular hyperfiltration by contraction of the afferent arteriole, dilation of the efferent arteriole, and by inhibition of tubular reabsorption. C-Peptide also plays a crucial role in renal pathophysiology. C-peptide binds to a cell surface receptor that is likely to be G-protein coupled. This interaction stimulates various cellular functions such as increasing intracellular Ca2+ concentration, enhancing PI3-K (phosphoinositide 3-kinase) activity, increased endothelial nitric oxide synthase (eNOS) transcription, and activation of the Na+/K+ ATPase and mitogen-activated protein kinase (MAPK) signaling pathway.

Kit Components Also Available Separately

Product No.
Description
SDS

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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C-Peptide: the missing link in diabetic nephropathy?
Nordquist L and Wahren J
The Review of Diabetic Studies : RDS, 6(3), 203-210 (2009)
Intracellular signalling by C-peptide.
Hills CE and Brunskill NJ
Experimental Diabetes Research (2008)
Human C-peptide antagonises high glucose-induced endothelial dysfunction through the nuclear factor-kappaB pathway.
Luppi P, et al.
Diabetologia, 51(8), 1534-1543 (2008)

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