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Key Documents

P1922

Sigma-Aldrich

Monoclonal Anti-Progesterone antibody produced in rat

clone 2H4, tissue culture supernatant

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rat

Quality Level

conjugate

unconjugated

antibody form

tissue culture supernatant

antibody product type

primary antibodies

clone

2H4, monoclonal

technique(s)

indirect ELISA: 1:1000 - 1:2000 (using Progesterone-BSA for coating.)
radioimmunoassay: suitable

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Progesterone or P4 (pregn-4-ene-3,20-dione) is a female steroid hormone that belongs to the hormones class, progestogens. It is produced by majorly by ovaries, adrenal glands and placenta of humans and other species. Progesterone derived from placenta and corpus luteum is required for the maintenance of pregnancy. Progesterone inhibits the T cell mediated immune response involved in tissue rejection. The progesterone levels in females are low before ovulation and are elevated during the luteal phase. Monoclonal Anti-Progesterone antibody is IgG1 isotype purified from rat cell culture supernatant.

Immunogen

progesterone-7-BSA.

Application

The antibody may be used in various immunochemical techniques including ELISA, radioimmunoassay AND RIA to measure progesterone levels in milk.

Analysis Note

dextran coated charcoal 3H RIA.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Letizia Debertolis et al.
Journal of veterinary science, 17(2), 189-197 (2015-12-10)
This study was conducted to evaluate the effects of induced endometritis on uterine blood flow in cows. Transrectal Doppler sonography was performed on uterine arteries of six cyclic cows before and for 4 days after inducing acute endometritis by intrauterine
Julia Jenikejew et al.
Conservation physiology, 9(1), coab098-coab098 (2022-01-07)
Progesterone and oestrogen are the main gonadal steroid hormones that regulate the ovarian activity and induce the fertile oestrus period in females. The monitoring of this receptive phase is particularly decisive for captive breeding and is commonly based on the
Kim Carnaby et al.
Reproduction (Cambridge, England), 144(4), 477-484 (2012-07-26)
Lynx presents a unique sexual cycle with persistent corpora lutea (CLs) and elevated serum progesterone (P₄) throughout parturition and lactation. In other mammals, CLs normally disintegrate after parturition, therefore the aim of our study was to characterise the annual life
Steroids.
N Applezweig
Chemical week, 104, 57-72 (1969-05-17)
J S Mitchell et al.
Journal of dairy science, 87(9), 2864-2867 (2004-09-18)
The objectives were to develop a novel protein conjugate-based ELISA test for whole milk progesterone with a dynamic range capable of fully profiling estrous cycles in the dairy cow and to study effects of whole milk medium on antibody binding

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