M7383
Malic Dehydrogenase from porcine heart
≥400 units/mg protein (biuret), ammonium sulfate suspension
Synonym(s):
L-Malate: NAD+ oxidoreductase, MDH, Malate Dehydrogenase
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About This Item
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Recommended Products
form
ammonium sulfate suspension
specific activity
≥400 units/mg protein (biuret)
foreign activity
Glutamic-Oxalacetic Transaminase ≤0.01%
Glutamic-Pyruvic Transaminase ≤0.01%
storage temp.
2-8°C
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General description
Cytoplasmic Isozyme
Application
Malic dehydrogenase has been used in a study to assess the use of a long-wavelength homogeneous enzyme immunoassay for the determination of amikacin in water samples. It has also been used in a study to investigate the lipid metabolism in a liver exposed to cadmium.
Biochem/physiol Actions
The surfactant cetyl (hexadecyl) trimethylammonium bromide inhibits the cytoplasmic isoform of porcine heart malate dehydrogenase but has no effect on the mitochondrial form. SDS however; inhibits both isoforms.
Unit Definition
One unit will convert 1.0 μmole of oxalacetate and β-NADH to L-malate and β-NAD per min at pH 7.5 at 25 °C.
Physical form
Suspension in 3.2 M (NH4)2SO4, 0.1 M KH2PO4, pH 7.0
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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K Smith et al.
Biochimica et biophysica acta, 884(1), 109-118 (1986-10-29)
The cationic surfactant, cetyl (hexadecyl) trimethylammonium bromide (CTAB), completely inactivates porcine heart cytoplasmic malate dehydrogenase (L-malate:NAD+ oxidoreductase, EC 1.1.1.37) at concentrations (of surfactant) which do not affect the activity of the mitochondrial isoenzyme. These concentrations are close to, or higher
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A simple and rapid homogeneous enzyme immunoassay involving the use of the malic dehydrogenase enzyme and a long-wavelength fluorophor, the oxazine Cresyl Violet, is proposed for the determination of the antibiotic amikacin in water samples. An enzymatic tracer has been
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