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C2488

Sigma-Aldrich

Citrate Buffer Solution, 0.09 M

Synonym(s):

Citric Acid Buffer Solution

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About This Item

UNSPSC Code:
12161700
eCl@ss:
32129211
NACRES:
NA.25

description

Contains 1% chloroform
pH 4.8 at 25 °C.

Quality Level

form

solution

concentration

0.09 M

pH

4.8 (25 °C)

application(s)

diagnostic assay manufacturing

storage temp.

2-8°C

General description

Citrate Buffer Solution, 0.09 M, is a high-quality biochemical buffer solution commonly employed in various research applications. Its applications include use in acid phosphatase reactions alongside p-nitrophenyl phosphate (pNPP) enzyme substrate. Citrate Buffer is also used for the isolation of RNA from cells and tissues. In addition, it is also used as a background electrolyte for separating sulfonamides utilizing capillary zone electrophoresis. With its superior buffering capacity and ability to maintain a stable pH, Citrate Buffer Solution, 0.09 M is an excellent choice for any researcher in need of reliable performance.

Application

Citrate Buffer Solution, 0.09 M has been used as a heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections during immunohistochemistry (IHC) experiments. It has also been used in the preparation of hybridization solutions for fluorescence in situ hybridization (FISH) procedures.

Other Notes

Unit definition: One unit of acid phosphatase will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per, minute at pH 4.8 at 37 °C.

Pictograms

Health hazard

Signal Word

Warning

Hazard Statements

Hazard Classifications

Carc. 2 - STOT RE 2 Oral

Target Organs

Liver,Kidney

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Migration behavior and separation of sulfonamides in capillary zone electrophoresis. I. Influence of buffer pH and electrolyte modifier
Lin CE, et al.
Journal of Chromatography A, 755(2), 261-269 (1996)
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