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Key Documents

38978

Sigma-Aldrich

Malachite Green solution

for microscopy

Synonym(s):

Basic Green 4, China Green, Victoria Green B, Aniline Green, Benzaldehyde green, CI 42000

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
MA.02

grade

for microscopy

Quality Level

product line

BioChemika

shelf life

limited shelf life, expiry date on the label

technique(s)

microbe id | staining: suitable

suitability

bacteria

SMILES string

OC(=O)C([O-])=O.CN(C)c1ccc(cc1)/C(c2ccccc2)=C3/C=C\C(C=C3)=[N+](/C)C

InChI

1S/C23H25N2.ClH/c1-24(2)21-14-10-19(11-15-21)23(18-8-6-5-7-9-18)20-12-16-22(17-13-20)25(3)4;/h5-17H,1-4H3;1H/q+1;/p-1

InChI key

FDZZZRQASAIRJF-UHFFFAOYSA-M

General description

Malachite Green is used for bacterial spore staining by Schaeffer and Fulton`s method. It can also be used in place of methyl green in the Pappenheim stain, when combined with the Gram stain.

Application

Staining of bacterial spores according to Schaeffer and Fulton; Pappenheim stain when combined with Gram′s stain

Components

Composition
Malachite green 10 g/l, in water (oxalate form is used)

replaced by

Hazard Statements

Hazard Classifications

Aquatic Chronic 3

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Randall Reif et al.
Nucleic acid therapeutics, 22(6), 428-437 (2012-11-02)
Due to the discovery of more and more roles of cellular noncoding RNAs, the approaches for introducing RNAs including small interfering RNA (siRNA), micro RNA (miRNA), ribozyme, and riboswitch into cells for regulating cell life cycle and for the treatment
Jia Zeng et al.
Biophysical journal, 104(1), 139-145 (2013-01-22)
It is shown that the nonlinear optical phenomenon known as second-harmonic generation can be used for label-free, time-resolved study of the transport of molecules through living cell membranes. The adsorption and transport of a 300-Da molecular-mass hydrophobic ion at the
S Nethaji et al.
Environmental science and pollution research international, 20(6), 3670-3678 (2012-11-08)
Char was obtained from lotus seed biomass by a simple single-step acid treatment process. It was used as an adsorbent for the removal of malachite green dye (MG) from simulated dye bath effluent. The adsorbent was characterized for its surface
Jagadeeswaran Chandrasekar et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(14), 5315-5320 (2013-03-20)
Catalytic DNA sequences (deoxyribozymes, DNA enzymes, or DNAzymes) have been identified by in vitro selection for various catalytic activities. Expanding the limits of DNA catalysis is an important fundamental objective and may facilitate practical utility of catalysts that can be
Jianwei Dong et al.
The Analyst, 137(19), 4571-4576 (2012-08-18)
A new surface-initiated polymerization based on pasting the initiator on a sensor chip surface was applied to prepare a malachite green (MG) imprinted ultrathin film on a surface plasmon resonance (SPR) sensor. First, the initiator (2,2-azoisobutyronitrile) was pasted on the

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