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UFC30GV0S

Millipore

Ultrafree® Centrifugal Filter, 0.5 mL Sample Volume

pore size 0.22 μm, PVDF membrane (hydrophilic)

Synonym(s):

Centrifuge filter

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About This Item

UNSPSC Code:
41104916
eCl@ss:
36100101
NACRES:
NB.24

material

PVDF membrane (hydrophilic)
polypropylene

Quality Level

400

sterility

sterile

feature

holdup volume 5 μL

manufacturer/tradename

Ultrafree®

parameter

0.5 mL sample volume
50 °C max. temp.

technique(s)

protein extraction: suitable
protein purification: suitable

L

45 mm

diam.

10.6 mm

filtration area

0.2 cm2

matrix

Durapore®

pore size

0.22 μm pore size

application(s)

sample preparation

shipped in

ambient

General description

Ultrafree®-MC (0.5 mL) and Ultrafree®-CL (2 mL) centrifugal devices are single-use, disposable filters used for removing particulates from aqueous biological solutions. These devices are available in two processing volumes with a range of microporous membranes (from 0.1 to 5.0 m) for fast filtration and highly reproducible performance. Pre-sterilized units are also available. Use in fixed-angle rotors for 1.5 mL tubes (-MC) and 15 mL tubes (CL).

Filter Code: GVPP
Filter Type: Microfiltration
Ultrafree®-MC (0.5 mL) and Ultrafree®-CL (2 mL) centrifugal devices are single-use, disposable filters used for removing particulates from aqueous biological solutions. These devices are available in two processing volumes with a range of microporous membranes (from 0.1 to 5.0 m) for fast filtration and highly reproducible performance. Pre-sterilized units are also available. Use in fixed-angle rotors for 1.5 mL tubes (-MC) and 15 mL tubes (CL).
Filter Code: GVPP
Filter Type: Microfiltration

Application

Ultrafree®-MC GV Centrifugal Filter has been used


  • to filter cell lysates after centrifugation for Label-free mass spectrometry
  • for filtering extracted enzymes in the buffer for preparing imaging solution for cellular direct stochastic optical reconstruction microscopy (dSTORM) and DNA-based point accumulation for imaging in nanoscale topography (DNA-PAINT) imaging
  • to purify viral solution during transfection studies
  • to filter heart tissue homogenates for preparation of the sample for high-pressure liquid chromatography (HPLC) analysis

Features and Benefits

  • Used for removal of particles and precipitates from aqueous and some solvent-based samples
  • Ideal for use with protein and nucleic acid solutions
  • Used for removal of particles and precipitates from aqueous and some solvent-based samples
  • Ideal for use with protein and nucleic acid solutions

Physical form

Color Code: Yellow

Legal Information

Durapore is a registered trademark of Merck KGaA, Darmstadt, Germany
Ultrafree is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Cetin Agar et al.
Blood, 117(25), 6939-6947 (2011-04-02)
Sepsis is a systemic host response to invasive infection by bacteria. Despite treatment with antibiotics, current mortality rates are in the range of 20%-25%, which makes sepsis the most important cause of death in intensive care. Gram-negative bacteria are a
Robert Player et al.
BMC genomics, 21(1), 166-166 (2020-02-19)
The state-of-the-art in nucleic acid based biodetection continues to be polymerase chain reaction (PCR), and many real-time PCR assays targeting biodefense pathogens for biosurveillance are in widespread use. These assays are predominantly singleplex; i.e. one assay tests for the presence
Beta2-glycoprotein I can exist in 2 conformations: implications for our understanding of the antiphospholipid syndrome.
Agar, Cetin, et al.
Blood, 116, 1336-1343 (2010)

Protocols

Affinity Purification with Ultrafree®-MC Centrifugal Filters

Affinity interaction chromatography is an effective method for protein purification that can achieve up to 95% purity in one step

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