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EZRL

Millipore

Rat Leptin ELISA Kit

measures and quantifies Leptin levels in 10 μL plasma or serum

Synonym(s):

Leptin, Obesity Factor

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.32

product name

Rat Leptin ELISA, This Rat Leptin ELISA is used to measure & quantify Leptin levels in Metabolism & Endocrine research.

Quality Level

species reactivity

rat

packaging

kit of 1 × 96 wells

parameter

10 μL sample volume (4hr assay)

assay range

accuracy: 102-122%
linearity: 87-100%
sensitivity: 0.04 ng/mL

technique(s)

ELISA: suitable

input

sample type plasma (K2 EDTA)
sample type serum

UniProt accession no.

application(s)

research use

detection method

colorimetric (450nm/590nm)

shipped in

wet ice

storage temp.

2-8°C

General description

Leptin is an adipokine secreted by the adipose tissue. It is also found in the primary and secondary lymphoid organs. Leptin has a 3D structure made up of four α-helix bundle motifs. It participates in regulating neuroendocrine function and energy homeostasis. Leptin also participates in physiological processes such as reproduction, angiogenesis, and hemopoiesis. Rat Leptin enzyme-linked immunosorbent assay (ELISA) kit is used for the non-radioactive quantification of leptin in rat sera. Plasma samples may also be used but application to samples of other biological fluids may need validation by the user.

Application

Rat Leptin ELISA kit has been used in the determination of leptin from rat serum and plasma samples.

Pictograms

CorrosionExclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Met. Corr. 1 - Skin Sens. 1

Storage Class Code

8A - Combustible corrosive hazardous materials


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Low-frequency electro-acupuncture and physical exercise improve metabolic disturbances and modulate gene expression in adipose tissue in rats with dihydrotestosterone-induced polycystic ovary syndrome.
Louise Manner?s, Ingibjorg H Jonsdottir, Agneta Holmang, Malin Lonn, Elisabet Stener-Victorin
Endocrinology null
Anxiety-like behaviour and associated neurochemical and endocrinological alterations in male pups exposed to prenatal stress.
Charlotte Laloux,Jerome Mairesse,Gilles Van Camp,Angela Giovine,Igor Branchi et al.
Psychoneuroendocrinology null
Bérengère Coupé et al.
PloS one, 7(1), e30616-e30616 (2012-02-01)
Intrauterine growth restriction (IUGR) is closely linked with metabolic diseases, appetite disorders and obesity at adulthood. Leptin, a major adipokine secreted by adipose tissue, circulates in direct proportion to body fat stores, enters the brain and regulates food intake and
Staci D Bilbo et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 24(6), 2104-2115 (2010-02-04)
Obesity is well characterized as a systemic inflammatory condition, and is also associated with cognitive disruption, suggesting a link between the two. We assessed whether peripheral inflammation in maternal obesity may be transferred to the offspring brain, in particular, the
Giuseppe Matarese et al.
Journal of immunology (Baltimore, Md. : 1950), 174(6), 3137-3142 (2005-03-08)
Leptin is an adipokine which conveys information on energy availability. In humans, leptin influences energy homeostasis and regulates neuroendocrine function primarily in states of energy deficiency. As a cytokine, leptin also affects thymic homeostasis and, similar to other proinflammatory cytokines

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