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Key Documents

ABS762

Sigma-Aldrich

Anti-NOTUM Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Protein notum homolog, NOTUM

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, human

technique(s)

immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable

UniProt accession no.

Q6P988

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... NOTUM(147111)

General description

Protein notum homolog (NOTUM) is expressed at a low level in most mammalian tissues, although it is overexpressed in a subset of human hepatocellular carcinomas. Its transcription is regulated by β-catenin/TCF and is a target of the Wnt signaling pathway, forming a negative feedback loop that regulates the expression and activity of the Wnt pathway. In Drosophila, the notum gene is regulated by the Wingless pathway and encodes a secreted hydrolase that modifies heparan sulfate proteoglycans. The mammalian homolog has been shown to be able to cleave glypicans and can release GPI-anchored proteins from the mammalian cell surface. Like the Drosophilia NOTUM, the mammalian protein.

Immunogen

Epitope: N-terminus
KLH-conjugated synthetic peptide corresponding to the N-terminus of human NOTUM.

Application

Anti-NOTUM Antibody is an antibody against NOTUM for use in western blotting, IHC & immunofluorescence.
Immunohistochemistry Analysis: 2.5 μg/mL from a representative lot detected NOTUM in human brain tissue.

Immunofluorescence Analysis: 20 μg/mL from a representative lot detected NOTUM in mouse kidney cells.
Research Category
Signaling
Research Sub Category
Developmental Signaling

Quality

Evaluated by Western Blotting in human kidney tissue lysate.

Western Blotting Analysis: 1 μg/mL of this antibody detected NOTUM in human kidney tissue lysate.

Target description

~73 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Physical form

Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Dogukan Mizrak et al.
Cell reports, 31(12), 107805-107805 (2020-06-25)
In the adult ventricular-subventricular zone (V-SVZ), neural stem cells (NSCs) generate new olfactory bulb (OB) neurons and glia throughout life. To map adult neuronal lineage progression, we profiled >56,000 V-SVZ and OB cells by single-cell RNA sequencing (scRNA-seq). Our analyses

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