Skip to Content
Merck
All Photos(3)

Documents

AB4326

Sigma-Aldrich

Anti-ISL-1 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

ISL LIM homeobox 1, ISL1 transcription factor, LIM/homeodomain, ISL1 transcription factor, LIM/homeodomain, (islet-1)

Sign Into View Organizational & Contract Pricing
All Photos(3)

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

horse, human, mouse

species reactivity (predicted by homology)

sheep (based on 100% sequence homology), canine (based on 100% sequence homology), rat (based on 100% sequence homology), equine (based on 100% sequence homology), bovine (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

NP_002193

UniProt accession no.

P61371

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ISL1(3670)

General description

Islet-1 (ISL-1) is a transcription factor that plays an important role in the embryogenesis of pancreatic islets of Langerhans. It is expressed in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain. In mouse embryos, a deficiency of this gene fail to undergo neural tube motor neuron differentiation. More recently it has been identified as a marker for a cardiac progenitor cell lineage that is capable of differentiating into all 3 major heart cell types: cardiomyocytes, smooth muscle and endothelial cell lineages.

Specificity

Predicted to react with most common vertebrate species based on 100% sequence homology.
This antibody recognizes ISL-1.

Immunogen

Epitope: Internal region of ISL-1.
KLH-conjugated linear peptide corresponding to human ISL-1.

Application

Anti-ISL-1 Antibody is a Rabbit Polyclonal Antibody for detection of ISL-1 also known as ISL LIM homeobox 1, ISL1 transcription factor LIM/homeodomain & has been validated in WB & IHC.
Immunohistochemistry Analysis: A 1:300 dilution from a previous lot detected ISL-1 in mouse pancreas tissue.

Quality

Evaluated by Western Blot in K562 cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected ISL-1 on 10 µg of K562 cell lysate.

Target description

~39 kDa

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Not finding the right product?  

Try our Product Selector Tool.

recommended

Product No.
Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Laura I Hudish et al.
Stem cell reports, 14(6), 1033-1043 (2020-05-11)
Human motor neuron (MN) diseases encompass a spectrum of disorders. A critical barrier to dissecting disease mechanisms is the lack of appropriate human MN models. Here, we describe a scalable, suspension-based differentiation system to generate functional human MN diseases in
Shuichi Kato et al.
Nagoya journal of medical science, 77(1-2), 253-263 (2015-03-24)
Transplantation of motoneurons (MN) into the peripheral nerve to provide a source of neurons for muscle reinnervation, termed motoneuron integrated striated muscle (MISM), may provide the potential to restore functional muscle activity, when combined with computer-programmed functional electrical stimulation (FES).
Joseph A Bisson et al.
Developmental biology, 398(1), 80-96 (2014-12-09)
Wnt proteins regulate cell behavior via a canonical signaling pathway that induces β-catenin dependent transcription. It is now appreciated that Wnt/β-catenin signaling promotes the expansion of the second heart field (SHF) progenitor cells that ultimately give-rise to the majority of
Rustam Esanov et al.
Experimental neurology, 277, 171-177 (2016-01-10)
Among several genetic mutations known to cause amyotrophic lateral sclerosis (ALS), a hexanucleotide repeat expansion in the C9orf72 gene is the most common. In approximately 30% of C9orf72-ALS cases, 5-methylcytosine (5mC) levels within the C9orf72 promoter are increased, resulting in
Karin Lauschke et al.
Stem cells and development, 30(7), 374-385 (2021-02-19)
The course of differentiation of pluripotent stem cells into cardiomyocytes and the intermediate cell types are characterized using molecular markers for different stages of development. These markers have been selected primarily from studies in the mouse and from a limited
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service