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AB15894

Sigma-Aldrich

Anti-Tbr2 Antibody

from chicken, purified by affinity chromatography

Synonym(s):

Eomesodermin homolog, T-box brain protein 2, T-brain-2, TBR-2, Tbr2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

chicken

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse

species reactivity (predicted by homology)

human, rat

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgY

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... EOMES(8320)

Related Categories

General description

Eomesodermin homolog (UniProt: O54839; also known as T-box brain protein 2, T-brain-2, TBR-2, Tbr2) is encoded by the Eomes (also known as Tbr2) gene (Gene ID: 13813) in murine species. T-box genes are a family of transcription factors have a highly conserved DNA binding domain named the T-domain. Tbr2 functions as a transcriptional activator that plays a crucial role during gastrulation, mesodermal specification, and limb patterning. It plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. It is also shown to be involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes. Its DNA-binding region is localized to amino acids 278-458. Conditional inactivation of Tbr2 during early brain development is known to cause microcephaly and severe behavior deficits. Two isoforms of Tbr2 have been described that are produced by alternative splicing. Isoform 2 lacks amino acids 463-481. (Ref.: Bulfone, A., et al. (1999). Mech. Dev. 84 (1-2); 133-138).

Specificity

This chicken polyclonal antibody specifically detects T-box brain protein 2 (Tbr2). It targets an epitope within 20 amino acids from the C-terminal region.

Immunogen

Epitope: Transcription Activation Domain
KLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal region of mouse T-box brain protein 2 (Tbr2).

Application

Anti-Tbr2, Cat. No. AB15894, is a chicken polyclonal antibody that detects Tbr2 and is tested for use in Western Blotting and Immunohistochemistry (Paraffin).
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience
Tested Applications

  • Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Tbr2 in Mouse brain tissue sections.
  • Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Quality

Evaluated by Western Blotting in E13-E14 Mouse brain tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Tbr2 in E13-E14 Mouse brain tissue lysate.

Target description

~72 and 74 kDa observed; 74.8 kDa calculated.. Uncharacterized bands may be observed in some lysate(s).

Physical form

Affinity purified
Purified chicken polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Recommended storage: +2°C to +8°C.

Analysis Note

Control
E13-14 mouse brain lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Tania A Seabrook et al.
Cell reports, 21(11), 3049-3064 (2017-12-16)
The use of sensory information to drive specific behaviors relies on circuits spanning long distances that wire up through a range of axon-target recognition events. Mechanisms assembling poly-synaptic circuits and the extent to which parallel pathways can "cross-wire" to compensate
Kazuhito Toyo-oka et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 34(36), 12168-12181 (2014-09-05)
During brain development, neural progenitor cells proliferate and differentiate into neural precursors. These neural precursors migrate along the radial glial processes and localize at their final destination in the cortex. Numerous reports have revealed that 14-3-3 proteins are involved in
Navneet A Vasistha et al.
Cerebral cortex (New York, N.Y. : 1991), 25(10), 3290-3302 (2014-06-15)
The individual contribution of different progenitor subtypes towards the mature rodent cerebral cortex is not fully understood. Intermediate progenitor cells (IPCs) are key to understanding the regulation of neuronal number during cortical development and evolution, yet their exact contribution is
Nereo Kalebic et al.
EMBO reports, 17(3), 338-348 (2016-01-14)
We have applied the CRISPR/Cas9 system in vivo to disrupt gene expression in neural stem cells in the developing mammalian brain. Two days after in utero electroporation of a single plasmid encoding Cas9 and an appropriate guide RNA (gRNA) into
Emily M Jobe et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 37(3), 523-536 (2017-01-20)
Methyl-CpG-binding domain 1 (MBD1) belongs to a family of methyl-CpG-binding proteins that are epigenetic "readers" linking DNA methylation to transcriptional regulation. MBD1 is expressed in neural stem cells residing in the dentate gyrus of the adult hippocampus (aNSCs) and MBD1

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