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G001719

HPLC Analysis of Carboxymethylated Apohemoglobin (Horse) Tryptic Digest on Discovery® BIO Wide Pore C18

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Discovery BIO Wide Pore C18, 15 cm × 2.1 mm I.D., 3 μm particles (568222-U)
mobile phase: [A] 0.1% TFA in water; [B] 0.1% TFA in water:0.1% TFA in acetonitrile, 50:50;
gradient: 0-100% B in 50 min
flow rate: 0.2 mL/min
column temp.: 35°C
detector: UV, 220 nm
injection: 5 μL, each at 0.8 mg/mL in 0.1% TFA

suitability

application for HPLC

application(s)

life science and biopharma

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description

    Apohemoglobin, carboxymethylated, tryptic digest

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