G001593
HPLC Analysis of Peptides on Discovery® BIO Wide Pore C18 (Gradient #2)
application for HPLC
About This Item
technique(s)
HPLC: suitable
test parameters
column: Discovery BIO Wide Pore C18, 15 cm × 4.6 mm I.D., 5 μm particles (568222-U)
mobile phase: [A] (80:20), 0.1%TFA in water:0.1% TFA in acetonitrile; [B] (66:34), 0.1% TFA in water:0.1% TFA in acetonitrile
flow rate: 1 mL/min
column temp.: 30 °C
detector: UV, 215 nm
injection: 5 μL Sigma Peptide Mix P2693 (~25 μg ea. Peptide) (~25 μg ea. peptide)
sample: Sigma Peptide Mix (Cat. No. P2693)
suitability
application for HPLC
application(s)
life science and biopharma
Legal Information
Analyte
analytical column
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