53591-U

Ascentis^® Express 90 Å F5 (2.7 μm) HPLC Columns

L × I.D. 15 cm × 4.6 mm, HPLC Column

• Synonym(s): Core-shell (SPP) Fused Core PFP HPLC column
• UNSPSC Code: 41115700
• eCl@ss: 32110501
• NACRES: SB.52

Properties

• product name: Ascentis^® Express F5, 2.7 μm HPLC Column, 2.7 μm particle size, L × I.D. 15 cm × 4.6 mm
• material: stainless steel column
• Agency: suitable for USP L43
• product line: Ascentis^®
• feature: endcapped
• manufacturer/tradename: Ascentis^®
• packaging: 1 ea of
• parameter: 60 °C temp. range; 600 bar max. pressure (9000 psi)
• technique(s): HPLC: suitable; LC/MS: suitable; UHPLC-MS: suitable; UHPLC: suitable
• L × I.D.: 15 cm × 4.6 mm
• surface area: 135 m²/g
• impurities: <5 ppm metals
• matrix: Fused-Core particle platform; superficially porous particle
• matrix active group: PFP (pentafluorophenyl) phase
• particle size: 2.7 μm
• pore size: 90 Å pore size
• operating pH: 2-8
• application(s): food and beverages
• separation technique: reversed phase

Description

General description

The pentafluorophenylpropyl stationary phase of Ascentis Express F5 provides a stable reversed phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines.In addition to forming pi-pi and mildly steric interactions, F5 phases also retain compounds by polar interactions. Ascentis Express F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.

Application

• Liquid chromatographic determination of lumacaftor in the presence of ivacaftor and identification of five novel degradation products using high-performance liquid chromatography ion trap time-of-flight mass spectrometry.: This study demonstrates the use of high-performance liquid chromatography (HPLC) with ion trap time-of-flight mass spectrometry for the determination of lumacaftor in the presence of ivacaftor. The research identifies five novel degradation products, showcasing the advanced analytical capabilities of the Ascentis^® Express F5, 2.7 µm HPLC Column for complex mixtures and degradation studies (Özcan et al., 2023).


• Stability-indicating LC-MS/MS and LC-DAD methods for robust determination of tasimelteon and high resolution mass spectrometric identification of a novel degradation product.: This paper details the development of stability-indicating methods using LC-MS/MS and LC-DAD for the determination of tasimelteon. It highlights the robustness and sensitivity of these methods in identifying novel degradation products, with the Ascentis^® Express F5 column playing a crucial role in separation efficiency and stability analysis (Özcan et al., 2020).


• Recent trends in ultra-fast HPLC: new generation superficially porous silica columns.: This review article discusses recent advancements in ultra-fast HPLC, focusing on new generation superficially porous silica columns, including the Ascentis^® Express F5, 2.7 µm HPLC Column. It provides insights into the enhanced performance, speed, and efficiency of these columns in high-throughput analytical applications (Ali et al., 2012).

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable