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ZRB1350

Sigma-Aldrich

Anti-IL-33 Antibody, clone 5K9, ZooMAb® Rabbit Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

Synonym(s):

IL-1F11, IL-33, Interleukin-1 family member 11, Interleukin-33, NF-HEV, Nuclear factor from high endothelial venules

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit (recombinant)

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

5K9, monoclonal
recombinant monoclonal

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 30.76 kDa
observed mol wt ~33 kDa

species reactivity

human, rat, mouse

packaging

antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

technique(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... IL33(90865)

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone 5K9 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Interleukin-33.

Immunogen

GST/His-tagged recombinant fragment corresponding to 146 amino acids from the C-terminal region of human IL-33.

Application

Anti-IL-33, clone 5K9 ZooMAb, Cat. No. ZRB1350, is a recombinant Rabbit monoclonal antibody that specifically targets IL-33 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), an Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected IL-33 in rat spleen, human spleen, EL4, and RAW264.7 cell lysates.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected IL-33 in human tonsil tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected IL-33 in RAW 264.7 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Interleukin-33 (UniProt: O95760; also known as IL-33, Interleukin-1 family member 11, IL-1F11, Nuclear factor from high endothelial venules, NF-HEV) is encoded by the IL33 (also known as C9orf26, IL1F11, NFHEV) gene (Gene ID: 90865) in human. IL-33 is a tissue-derived nuclear cytokine of the IL-1 family that is highly expressed in endothelial cells, epithelial cells and fibroblast-like cells. It is normally released from damaged and necrotic barrier cells and serves as an alarmin to promote activation of the innate immune system and removal of pathogens. It is considered as a ubiquitous immune modulator. IL-33 is expressed at high levels in endothelial venules in tonsils, Peyer patches, and mesenteric lymph nodes. It exerts its cytokine activity by forming a 1:1:1 heterotrimeric complex with its primary high-affinity receptor IL1RL1 and the co-receptor IL1RAP. This complex activates NF-kappa B and MAP kinase signaling in target cells. IL-33 is also reported to be involved in the maturation of Th2 cells and inducing the secretion of T-helper type 2-associated cytokines. Although full-length IL-33 can signal via IL1R1 receptor, proteolytic processing by cathepsin G and neutrophil elastase produces C-terminal peptides that are shown to be more active than the unprocessed full-length protein. IL-33 can also undergo proteolytic cleavage by caspase-3 and -7 that results in its activation. IL-33 has a homeodomain-like HTH domain (aa 1-65) that mediates its nuclear localization and heterochromatin association. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Liew, FY., et al. (2016). Nat. Rev. Immunol. 16; 676-689; Carrol, C., and Girard, JP (2018). Immunol. Rev. 281(1); 154-168).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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