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Key Documents

SRP8024

Sigma-Aldrich

TRAIL, Soluble human

recombinant, expressed in E. coli, His tagged, >98% (SDS-PAGE)

Synonym(s):

Apo-2L, CD253, CD339, TRAIL

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.77

biological source

human

recombinant

expressed in E. coli

tag

His tagged

Assay

>98% (SDS-PAGE)

form

liquid

mol wt

~26 kDa

packaging

pkg of 20 μg

concentration

≥0.2 mg/mL

technique(s)

activity assay: suitable
cell culture | mammalian: suitable
transfection: suitable

impurities

<0.01 EU/μg endotoxin, tested

color

clear

UniProt accession no.

P50591

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... TNFSF10(8743)

General description

TNF-related apoptosis-inducing ligand (TRAIL; Apo2L; CD253; TNFSF10) is a type II transmembrane protein of about 34kDa. Like most members of the tumor necrosis factor (TNF) superfamily of cytokines, TRAIL can be cleaved at the cell surface by metalloproteases to form a soluble molecule. The gene encoding TRAIL is localized on human chromosome 3q26.31.

Application

TRAIL, soluble human has been used in cell culture, chemical transfection and in caspase-3/7 activity assay.

Biochem/physiol Actions

Trimerized TNF-related apoptosis-inducing ligand (TRAIL) triggers apoptosis upon ligation of cell surface TRAIL-receptor 1 (R1) and/or TRAIL-R2 by inducing the formation of the so-called multiprotein death-inducing signaling complex (DISC). Active TRAIL forms trimers and specifically binds to five distinct receptors: TRAIL-receptors 1,-2,-3,-4 {TRAIL-R1 (DR4; Apo2; CD261; TNFRSF10A), TRAIL-R2 (DR5; KILLER; TRICK2A; TRICK2B; CD262; TNFRSF10B), TRAIL-R3 (DcR1; LIT; TRID; CD263; TNFRSF10C), TRAIL-R4 (DcR2; TRUNDD; CD264; TNFRSF10D)} and osteoprotegerin (OPG; OCIF; TNFRSF11B).

Physical form

Solution in 20 mM HEPES, pH 7.9, 120 mM NaCl, 0.01% Tween 20, 1% sucrose, 1 mM DTT.

Other Notes

The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a His-tag and a linker peptide. The active multimeric conformation is stabilized by an inserted mutation allowing an additional CC-bridge.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Genome-Scale MicroRNA and Small Interfering RNA Screens Identify Small RNA Modulators of TRAIL-Induced Apoptosis Pathway
Dmitriy Ovcharenko
Cancer Research (2007)
Association of TRAIL and Its Receptors with Large-Artery Atherosclerotic Stroke.
Pan X
PLoS ONE, 10(9), e0136414-e0136414 (2015)
Intracranial AAV-sTRAIL combined with lanatoside C prolongs survival in an orthotopic xenograft mouse model of invasive glioblastoma.
Crommentuijn MH
Molecular Oncology, 10(4), 625-634 (2016)
Association between endometriosis and polymorphisms in tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), TRAIL receptor and osteoprotegerin genes and their serum levels.
Kim H
Archives of Gynecology and Obstetrics, 286(1), 147-153 (2012)
Association of Polymorphisms in TRAIL and Chronic Hepatitis B in Chinese Han Populations from Shandong Province.
Wang F
Annals of Clinical and Laboratory Science, 46(4), 374-379 (2016)
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