SRP0395
SETD2 active human
recombinant, expressed in E. coli, ≥70% (SDS-PAGE)
Synonym(s):
HYPB, SET domain containing 2, SET2
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
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biological source
human
recombinant
expressed in E. coli
Assay
≥70% (SDS-PAGE)
form
aqueous solution
mol wt
60.9 kDa
packaging
pkg of 50 μg
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... SETD2(29072)
General description
SET domain containing 2 (SETD2) is a histone methyltransferase gene and is mapped on human chromosome 3p21.31, a region associated with growth arrest of cancer cells.
Human SETD2/pGEX6P1 (GenBank Accession No. NM_014159), amino acids 1418-1714 with N-terminal GST-tag, MW= 60.9kDa, expressed in an Escherichia coli expression system.
Human SETD2/pGEX6P1 (GenBank Accession No. NM_014159), amino acids 1418-1714 with N-terminal GST-tag, MW= 60.9kDa, expressed in an Escherichia coli expression system.
Biochem/physiol Actions
SET domain containing 2 (SETD2) plays a vital role in trimethylation of the histone mark H3K36 (histone 3 lysine 36). Mutation of this tumor suppressor gene (TSG) is associated with the pathogenesis of various cancers including, clear cell renal cell carcinoma (cRCC), breast cancer, lung cancer, acute lymphoblastic leukemia(ALL), and glioma. In addition, SETD2 gene is also involved in the development of Huntington′s disease (HD). SETD2 plays an important role in the transcriptional elongation by maintaining chromatin structure through association with hyperphosphorylated RNA polymerase II (POLR2A). SETD2 interacts with p53 and act as a tumor suppressor in breast cancer cells. SETD2 is implicated in DNA double-strand break (DSB) exclusion and homologous recombination (HR) repair in human cells. SETD2 in HeLa cells alters nucleosome organization via decreasing the recruitment of the FACT (facilitates chromatin transcription) complex to active genes.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Histone methyltransferase SETD2 coordinates FACT recruitment with nucleosome dynamics during transcription.
Carvalho S
Nucleic Acids Research, 41(5), 2881-2893 (2013)
Histone methyltransferase gene SETD2 is a novel tumor suppressor gene in clear cell renal cell carcinoma.
Duns G
Cancer Research, 70(11), 4287-4291 (2010)
The mRNA expression of SETD2 in human breast cancer: correlation with clinico-pathological parameters.
Al Sarakbi W
BMC Cancer, 9:290 (2009)
S Rega et al.
Molecular and cellular neurosciences, 18(1), 68-79 (2001-07-20)
Neurodegeneration in Huntington's disease (HD) is associated with an elongated glutamine tract in the widely expressed huntingtin protein. Although the pathogenic mechanisms are still unknown, the distinct physical properties of mutant huntingtin in the brain suggest that other factors including
SETD2-dependent histone H3K36 trimethylation is required for homologous recombination repair and genome stability.
Pfister SX
Cell Reports, 7(6), 2006-2018 (2014)
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