SRE0112
Heat-labile Cod Uracil-DNA Glycosylase
recombinant, expressed in E. coli, Triton-free and Glycerol-free
Synonym(s):
UDG, UNG
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About This Item
UNSPSC Code:
41106300
NACRES:
NA.54
Recommended Products
recombinant
expressed in E. coli
Quality Level
composition
Triton-free and Glycerol-free
shipped in
dry ice
storage temp.
−20°C
General description
Uracil-DNA glycosylases are DNA repair enzymes that participate in the base excision repair pathway. UracilDNA Glycosylase, also known as UNG or UDG, is an enzyme that hydrolyses the N-glycosylic bond between the deoxyribose sugar and the base in uracil-containing DNA leaving an abasic (apyrimidinic) site in DNA. Contamination of PCR samples with amplicon DNA generated by previous reactions, “carry-over contamination”, is one of the major sources of false positive results. Heat-labile Cod Uracil-DNA Glycosylase prevents carry-over contamination in PCR, qPCR, RT-qPCR, and RT-LAMP assays. The enzyme is produced in a recombinant E.coli (ung–) strain that contains a modified Cod UNG gene.
Specificity
One Unit is defined as the amount of enzyme that liberates 1 nmol Uracil per hour from Uracil-labelled DNA at 37°C in 70 mm Tris-HCI pH 8.0 (@25°C), 10 mM NaCI, 1 mM EDTA and 0.1 mg/ml BSA.
Application
Heat-labile Cod Uracil-DNA Glycosylase eliminates carry-over contamination in PCR, RT-PCR, RT-qPCR, qPCR and RT-LAMP
Features and Benefits
- Heat inactivation: The only UNG that is completely and irreversibly heat inactivated thus ensuring long-term sample integrity for subsequent post-PCR analysis such as cloning, sequencing or genotyping
- Cod UNG is not active on uracil in RNA.
- High sensitivity: Remove more than 108 copies of contaminating DNA without affecting the sensitivity (Cq) of the assay
- Simple protocol: Applying Cod UNG in a RT-PCR is done by a 5 min Cod UNG preincubation step at RT prior to the amplification reaction
Reconstitution
In solution
Other Notes
This product is for R&D use only. Not for drug, household, or other uses.
related product
Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Find documentation for the products that you have recently purchased in the Document Library.
Uracil-DNA glycosylases?structural and functional perspectives on an essential family of DNA repair enzymes
Schormann N, et al.
Protein Science, 23(12), 1667-1685 (2014)
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