SAE0150
Chondroitinase ABC from Proteus vulgaris
recombinant, low endotoxin, aqueous solution, ≥100 U/ml, 50-250 units/mg protein, BSA free
Synonym(s):
Ch′ase ABC, Chondroitin ABC Lyase
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.77
Recommended Products
biological source
bacterial (Proteus vulgaris)
Quality Level
conjugate
(Glucosaminoglycan)
specific activity
50-250 units/mg protein
shipped in
dry ice
storage temp.
−70°C
Related Categories
General description
Chondroitinase ABC is a lyase with specificity toward chondroitin sulfates and dermatan sulfates - with some minor activity toward hyaluronan. This recombinant version only contains the endolytic chondroitinase, meaning it will not digest the non-reducing ends of proteoglycan substrates. It targets β-(1,4)-linkages between N-acetylgalactosamine and either D-glucuronic acid or L-iduronic acid. The product of digestion is a mixture of Δ 4-unsaturated tetra- and di-saccharides.
Application
- Degradation of Chondroitin sulfate-containing tissue such as extracellular matrices and cartilage
- Neural regeneration research
- Eliminative degradation of all forms of Chondroitin sulfate: A, B and C
- Quantitation of glucosaminoglycans (mucopolysaccharides)
Features and Benefits
- Low Endotoxin
- BSA Free
- Recombinant
- Endolytic component only, does not contain exolytic enzyme.
- Compatible with chondroitin 4-sulfate, chondroitin 6-sulfate, and dermatan sulfate, and acts slowly on hyaluronate
Components
Non active Ingredients: Hepes-Sodium salt, NaCl, Trehalose, Imidazole, Glycerol, Water
Compatibility
pH optimum:
Temperature optimum: 37 °C
Activator: 0.05 M acetate
Inhibitors: 1 mM Zn2+ Ni2+, Fe2+ and Cu2+
- pH 8.0 (chondroitin sulfate)
- pH 6.8 (hyaluronic acid)
Temperature optimum: 37 °C
Activator: 0.05 M acetate
Inhibitors: 1 mM Zn2+ Ni2+, Fe2+ and Cu2+
Unit Definition
1 unit will liberate 1.0 μmole of unsaturated disaccharides per min at pH 8.0 at 37 °C, using chondroitin sulfate from shark cartilage (C4384) as substrate.
Storage and Stability
Store the product at -70 °C. When stored unopened at -70 °C, the buffer is stable for at least two years.
Other Notes
This product is for R&D use only. Not for drug, household, or other uses. Please consult the SafetyData Sheet for information regarding hazards and safe handling practices.
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Certificates of Analysis (COA)
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Find documentation for the products that you have recently purchased in the Document Library.
Functions of Chondroitin Sulfate and Heparan Sulfate in the Developing Brain
Maeda, N., et al.
Neurochemical Research, 1228-1240 (2011)
Two distinct chondroitin sulfate ABC lyases an endoeliminase yielding tetrasaccharides and an exoeliminase preferentially acting on oligosaccharides
Hamai, A. et al.
The Journal of Biological Chemistry, 9123-9130 (1997)
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