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S0638

Sigma-Aldrich

Serum Replacement 1 (50×)

liquid, sterile-filtered, suitable for cell culture

Synonym(s):

50× Serum Replacement, Cell Culture Serum Substitute, Replacement Serum

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.71

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

storage temp.

2-8°C

Application

Serum Replacement 1 (50×) has been used as a supplement in Ham′s/F-12 medium for tumour cells. It has also been used as a supplement to culture Jurkat T cells in RPMI medium.(2)

Other Notes

Defined, multi-purpose serum replacement useful in studying the effects of growth factors and the production of proteins. Contains highly purified, heat-treated bovine serum albumin, heat-treated bovine transferrin and bovine insulin. Does not contain growth factors, steroid hormones, glucocorticoids, cell adhesion factors, detectable Ig and mitogens. May be used with anchorage-dependent and suspension cells.

Caution

Stable for 30 days when diluted in cell culture medium.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Proliferation and intracellular glutathione in Jurkat T cells with concentrated whey protein products
Middleton N, et al.
International Journal of Dairy Technology, 13(7), 565-573 (2003)
Constanze M Hammerle et al.
PLoS genetics, 16(10), e1009069-e1009069 (2020-10-16)
The genetic mechanisms that determine the size of the adult pancreas are poorly understood. Imprinted genes, which are expressed in a parent-of-origin-specific manner, are known to have important roles in development, growth and metabolism. However, our knowledge regarding their roles

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