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Key Documents

RAB0654

Sigma-Aldrich

Human EPO / Erythropoietin ELISA Kit

for serum, plasma, cell culture supernatants and urine

Synonym(s):

Erythropoietin

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable

input

sample type cell culture supernatant(s)
sample type plasma
sample type serum
sample type urine

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 0.15 mIU/mL
standard curve range: 0.137-100 mIU/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... EPO(2056)

General description

The antibody pair provided in this kit recognizes Human Erythropoietin.

Application

Human EPO / Erythropoietin ELISA Kit has been used to measure concentration of erythropoietin (EPO).
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Erythropoietin (EPO) plays a vital role in the regulation of red blood cell production. It interacts with EPO receptor and is responsible for the cellular responses. EPO is expressed in various types of cancers, such as breast cancer, renal cancer, gastric cancer, hepatocellular carcinoma and central nervous system tumors. EPO also acts as a hypoxia responsive cytokine and a pro-angiogenic factor. This protein provides major neuroprotective effect against brain damages due to ischemia and enhances memory as well as cognitive ability in humans suffering from vascular dementia.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS

  • RABELADDELISA 5X Assay/Sample Diluent Buffer D (Item K)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Eunhee Han et al.
Clinica chimica acta; international journal of clinical chemistry, 464, 113-117 (2016-11-20)
Antimitochondrial antibody (AMA) is a specific serologic marker in primary biliary cirrhosis (PBC). The aim of this study was to evaluate the clinical relevance of combined AMA assays. Sera were obtained from 79 patients with PBC and 108 patients with
Hussam Ghoti et al.
The American journal of pathology, 190(10), 2146-2154 (2020-08-04)
Patients with thalassemia exhibit an increased risk of thrombotic events that is augmented after splenectomy. Heparanase protein enhances cancer progression, angiogenesis, and inflammation; it also activates the coagulation system through direct interaction with tissue factor (TF). Additionally, erythropoietin, which is
Shaohong Cheng et al.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 29(10), 2276-2286 (2014-04-23)
We have previously shown that the increase in osterix (Osx) expression during osteoblast maturation is dependent on the activity of the prolyl hydroxylase domain-containing protein 2 (Phd2), a key regulator of protein levels of the hypoxia-inducible factor family proteins in
Essi V Koskela et al.
Biotechnology journal, 12(8) (2017-04-22)
Therapeutic protein production in yeast is a reality in industry with an untapped potential to expand to more complex proteins, such as full-length antibodies. Despite numerous engineering approaches, cellular limitations are preventing the use of Saccharomyces cerevisiae as the titers
Shun-Tao Luo et al.
Scientific reports, 7, 38105-38105 (2017-02-07)
The simultaneous increases in blood lactic acid and erythrocytes after intense exercise could suggest a link between lactate and the erythropoiesis. However, the effects of lactic acid on erythropoiesis remain to be elucidated. Here, we utilized a mouse model to

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