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RAB0016

Sigma-Aldrich

Human Angiopoietin-2 ELISA Kit

for serum, plasma, cell culture supernatant and urine

Synonym(s):

Angiopoietin-2

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type urine
sample type serum
sample type cell culture supernatant(s)
sample type plasma

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 10 pg/mL
standard curve range: 4.12-3000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... ANGPT2(285)

Related Categories

General description

The Human Angiopoietin-2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Angiopoietin-2 in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human Angiopoietin-2

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Angiopoietin-2 (ANGPT2) belongs to the family of angiogenic factors. ANGPT2 is a competitive antagonist of ANGPT1 and reduces vascular stabilization. ANGPT2 is a potent marker for liver cancer. ANGPT2 binds with tyrosine kinase with immunoglobulin-like and endothelial growth factor (EGF)-like domains 2 (Tie-2) and causes neoangiogenesis in liver cirrhosis. Upregulation of ANGPT2 might have an adverse effect on kidney physiology and function. Overexpression of ANGPT2 leads to endothelial damage and dysfunction resulting in diabetic nephropathy in diabetic patients. ANGPT2 is a potent marker for detecting lung cancer progression. Upregulation of ANGPT2 promotes signal transduction for tumor progression in epithelial ovarian cancer.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS
  • RABELADEELISA 5X Assay/Sample Diluent Buffer E (Item E2)SDS
  • RABSTOP3ELISA Stop Solution (Item I)SDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS
  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Crosstalk between TEMs and endothelial cells modulates angiogenesis and metastasis via IGF1-IGF1R signalling in epithelial ovarian cancer
Wang X, et al.
British Journal of Cancer, 117(9), 1371-1382 (2017)
The role of serum angiopoietin-2 levels in progression and prognosis of lung cancer: A meta-analysis
Xu Y, et al.
Medicine, 96(37), e8063-e8063 (2017)
Angiopoietin-2, Renal Deterioration, Major Adverse Cardiovascular Events and All-Cause Mortality in Patients with Diabetic Nephropathy
Tsai YC, et al.
Kidney & Blood Pressure Research, 43, 545-554 (2018)
Angiopoietin-2 (Ang-2) is a useful serum tumor marker for liver cancer in the Chinese population
Chen Y, et al.
Clinica Chimica Acta; International Journal of Clinical Chemistry, 478, 18-27 (2018)
Angiopoietin-2 serum levels are elevated in patients with liver cirrhosis and hepatocellular carcinoma
Scholz A, et al.
The American Journal of Gastroenterology, 102(11), 2471-2481 (2007)

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