P2621

Phosphomannose Isomerase from Escherichia coli

recombinant, expressed in E. coli, ammonium sulfate suspension, ≥50 units/mg protein

• Synonym(s): D-Mannose-6-phosphate ketol-isomerase, Mannose Phosphate Isomerase, PMI
• Enzyme Commission number: 5.3.1.8 (BRENDA, IUBMB)
• MDL number: MFCD00131899
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• recombinant: expressed in E. coli
• Quality Level: 200
• form: ammonium sulfate suspension
• specific activity: ≥50 units/mg protein
• storage temp.: 2-8°C

Description

Application

PMI is used to study cell wall synthesis and energy production. PMI has been used to study how EDTA and metal ions, such as Zn++, Co++, Fe++, Mn++ and Cu++., can affect recovery and thermal stability. It may be used to study PMI′s effect on various alginate biosynthetic enzymes such as phosphomannomutase (PMM), GDP-mannose pyrophosphorylase (GMP), and GDP-mannose dehydrogenase (GMD).

Biochem/physiol Actions

Phosphomannose Isomerase (PMI) catalyses the interconversion of mannose 6-phosphate (Man-6-P) and fructose 6-phosphate (Fru-6-P), which provides a link between glucose metabolism and mannosylation.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Unit Definition

One unit will convert 1.0 μmole of D-mannose 6-phosphate to D-fructose 6-phosphate per min at pH 7.6 at 25 °C, using a coupled enzyme system with phosphoglucose isomerase and glucose-6-phosphate dehydrogenase.

Physical form

Supplied as a suspension in 3.2 M ammonium sulfate

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves