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P0044

Sigma-Aldrich

Phosphatase Inhibitor Cocktail 3

DMSO solution, for the inhibition of protein phosphatase 2A, alkaline phosphatases, protein phosphatases 1 and 2A, DMSO solution

Synonym(s):

Phosphatase inhibitor

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About This Item

EC Number:
200-664-3
UNSPSC Code:
12352200
NACRES:
NA.77
form:
DMSO solution
solubility:
water: soluble
storage temp.:
2-8°C
Pricing and availability is not currently available.

Product Name

Phosphatase Inhibitor Cocktail 3, DMSO solution

form

DMSO solution

Quality Level

200

solubility

water: soluble

shipped in

wet ice

storage temp.

2-8°C

Related Categories

Application

The phosphatase inhibitor cocktail improves the yields of intact proteins by adding inhibitors to enzymes that modify proteins present in cell extracts. This product has been tested on extract from animal tissues including human placenta and bovine liver, and on extracts of A431, CHO, and U937 cells. It has been optimized and tested for L-isozymes of alkaline phosphatase as measured with pNPP and for serine-threonine protein phosphatases.

Biochem/physiol Actions

This mixture contains individual components with specific inhibitory properties. Cantharidin inhibits protein phosphatase 2A. (-)-p-Bromolevamisole oxalate inhibits L-isoforms of alkaline phosphatases. Calyculin A inhibits protein phosphatases 1 and 2A.

Caution

As supplied, this product is stable for four years at the recommended 2-8°C storage temperature.

Preparation Note

This cocktail is supplied as a clear solution in dimethyl sulfoxide. It is recommended to dilute the cocktail at 1 mL of cocktail per 500 mg of protein extracted from tissue or cells. Often a final concentration of 1% (v/v) is used, which will inhibit phosphatase activities found in the 27,000 x g supernatant from human placenta and bovine liver, and in the 100,000 x g supernatant from A431, CHO, and U937 extracts.

Other Notes

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

188.6 °F

Flash Point(C)

87 °C

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Certificates of Analysis (COA)

Lot/Batch Number
0000398626
0000403121
0000312680
0000312680
0000398626

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Questions

1–3 of 3 Questions  

  1. Does this reagent contain EDTA or EGTA or similar?

    1 answer

    1. This product does not contain any chelating agents, including EDTA and EGTA.

      Helpful?

  2. What's the concentration of this reagent?

    1 answer

    1. The concentration of the individual components are proprietary. See below for the inhibitory properties of each:
      • Cantharidin: inhibits protein phosphatase 2A (PP-2A)
      • (–)-p-Bromolevamisole oxalate: inhibits L-isoforms of alkaline phosphatases
      • Calyculin A: inhibits protein phosphatases 1 and 2A (PP-1 and PP-2A)

      Please see the link below to review the full product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/306/088/p0044dat-ms.pdf

      Helpful?

  3. Does this product contain any metal chelators?

    1 answer

    1. This phosphatase inhibitor cocktail does not contain a chelator. A phosphatase inhibitor cocktail is not offered that includes EDTA at this time. Other protease inhibitor cocktails with chelators are offered.

      Helpful?

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