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Key Documents

N5264

Sigma-Aldrich

Monoclonal Anti-Neurofilament 160 antibody produced in mouse

clone NN18, ascites fluid

Synonym(s):

Monoclonal Anti-Neurofilament medium chain

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

NN18, monoclonal

mol wt

antigen 160 kDa

contains

15 mM sodium azide

species reactivity

human, pig

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40 using human tissue sections
immunohistochemistry (frozen sections): suitable
western blot: suitable

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

chicken ... NEFM(396206)
human ... NEFM(4741)
mouse ... Nefm(18040)
rat ... Nefm(24588)

General description

Monoclonal Anti-Neurofilament 160 (mouse IgG1 iso-type) is produced by the fusion mouse myeloma cells and splenocytes from an immunized mouse. Neurofilaments are composed of three sub-units- light NFL protein, medium NFM protein, heavy NFH protein. Along with there two other intermediate filaments are present α-internexin and peripherin. Neurofilaments are phosphoproteins.
Neurofilaments belong to the intermediate filament family and are expressed mainly in cells or tissues of neuronal origin. It is crucial for proper radial growth of axons.

Specificity

Mouse monoclonal anti-neurofilament 160 antibody reacts specifically with neurofilaments of molecular weight 160,000 but does not cross react with other intermediate filament proteins.

Immunogen

neurofilaments from pig spinal cord.

Application

Monoclonal anti-neurofilament 160 antibody (diluted 1: 5000) can be used as internal loading controls in immunoblotting. It can also be used in whole-mount immunohistochemistry and western blot. Monoclonal anti-neurofilament 160 antibodies can be used for localization of neurofilaments of molecular weight 160,000 in cultured cells or tissues preparation.
Monoclonal anti-neurofilament 160 antibody has been used in
  • Immunoblotting.
  • Immunohistochemistry
  • Immunofluorescence

Biochem/physiol Actions

Neurofilaments play a role in axonal calibre as they help in movement of an impulse down the axon. Their activity depends on phosphorylation of neurofilaments.Mutations of neurofilaments causes Charcot-Marie-Tooth (CMT) disease. Accumulation of neurofilaments has been observed in many human neurological diseases like Alzheimer′s disease, progressive supranuclear palsy, diabetic neuropathy, and giant axonal neuropathy.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Svetlana Gorokhova et al.
PLoS genetics, 10(2), e1004081-e1004081 (2014-02-12)
Neurotrophins and their receptors control a number of cellular processes, such as survival, gene expression and axonal growth, by activating multiple signalling pathways in peripheral neurons. Whether each of these pathways controls a distinct developmental process remains unknown. Here we
Peripheral nerve regeneration monitoring using multilayer microchannel scaffolds.
Yoonsu Choi et al.
Neural regeneration research, 11(3), 422-423 (2016-04-30)
F Maina et al.
Genes & development, 11(24), 3341-3350 (1998-02-07)
The development of the nervous system is a dynamic process during which factors act in an instructive fashion to direct the differentiation and survival of neurons, and to induce axonal outgrowth, guidance to, and terminal branching within the target tissue.
Hak Hyun Ryu et al.
Journal of veterinary science, 10(4), 273-284 (2009-11-26)
In this study, we evaluated if the implantation of allogenic adipose-derived stem cells (ASCs) improved neurological function in a canine spinal cord injury model. Eleven adult dogs were assigned to three groups according to treatment after spinal cord injury by
Tiziana Bisogno et al.
The Journal of cell biology, 163(3), 463-468 (2003-11-12)
Diacylglycerol (DAG) lipase activity is required for axonal growth during development and for retrograde synaptic signaling at mature synapses. This enzyme synthesizes the endocannabinoid 2-arachidonoyl-glycerol (2-AG), and the CB1 cannabinoid receptor is also required for the above responses. We now

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