N3129

4-Nitrophenyl phosphate bis(cyclohexylammonium) salt

phosphatase substrate, chromogenic, ≥97% anhydrous basis, powder

• Synonym(s): p-Nitrophenyl phosphate bis(cyclohexylammonium) salt, PNPP
• Linear Formula: O₂NC₆H₄OP(O)(O^-)₂ · 2C₆H₁₁NH₃⁺
• CAS Number: 52483-84-8
• Molecular Weight: 417.44
• NACRES: NA.83
• PubChem Substance ID: 24897623
• UNSPSC Code: 12352204
• EC Number: 257-949-0
• MDL number: MFCD00013173
• Beilstein/REAXYS Number: 6553276

Properties

• Product Name: 4-Nitrophenyl phosphate bis(cyclohexylammonium) salt, phosphatase substrate
• Quality Segment: 300
• assay: ≥97% anhydrous basis
• form: powder
• solubility: water: 10 mg/mL, clear, colorless to faintly yellow
• storage temp.: −20°C
• SMILES string: NC1CCCCC1.NC2CCCCC2.OP(O)(=O)Oc3ccc(cc3)[N+]([O-])=O
• InChI: 1S/C6H6NO6P.2C6H13N/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*7-6-4-2-1-3-5-6/h1-4H,(H2,10,11,12);2*6H,1-5,7H2
• InChI key: KXNNUYCXODNFBD-UHFFFAOYSA-N

Description

Application

4-Nitrophenyl phosphate bis(cyclohexylammonium) salt has been used as a component in the colorimetric reagent for abscisic acid (ABA) assay.

Biochem/physiol Actions

p-Nitrophenyl phosphate (pNPP) is the preferred substrate for use with alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures. Due to its high sensitivity, pNPP is the considered substrate in alkaline phosphatase systems. ELISA applications based on pNPP can be read in timed assays or delayed readings by stopping with alkaline solutions. This substrate yields a yellow-colored soluble end product that can be measured spectrophotometrically at 405 nm.

Safety Information

• Storage Class: 11 - Combustible Solids
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)