MAK443
L-Lactate Assay Kit
Sufficient for 100 fluorometric tests
Synonym(s):
ʟ-Lactic Acid Assay Kit
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Recommended Products
Quality Level
input
cell culture medium
plasma
serum
urine
detection method
fluorometric
relevant disease(s)
diabetes; pulmonary disorders; endocrinological disorders; cardiovascular diseases; cancer
storage temp.
−20°C
General description
Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology.
Application
The L-Lactate Assay Kit may be used for:
- Lactate Metabolism Research
- Tissue Metabolism Research
- Drug Discovery
Features and Benefits
- Simplified Process: Experience a streamlined process with the addition of only a single working reagent, reducing complexity and saving valuable time and effort.
- Broader Detection Range: Detect L-Lactate levels accurately across a wide range, from 1 to 50 μM, using just 50 μL of sample in a 96-well plate setup, ensuring comprehensive analysis with minimal sample requirement.
- High-Throughput Compatibility: Effortlessly integrate our kit into high-throughput handling systems, ensuring seamless operation and maintaining efficiency and accuracy in large-scale analyses.
Suitability
The kit is suitable for the quantitative determination of L-lactate (L-lactic acid) and the evaluation of drug effects on its metabolism in serum, plasma, urine, cell culture media, and other biological samples.
Principle
The L-Lactate Assay Kit is based on the lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λEx = 530 nm/λEm = 585 nm, is proportional to the lactate concentration in the sample.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
related product
Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service