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M7659

Myosin Heavy Chain from rabbit muscle

buffered aqueous glycerol solution

Synonym(s):

ATPase inactive whole chain myosin

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About This Item

CAS Number:
UNSPSC Code:
12352202
NACRES:
NA.32
MDL number:
Form:
buffered aqueous glycerol solution
Biological source:
rabbit muscle
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biological source

rabbit muscle

form

buffered aqueous glycerol solution

technique(s)

electrophoresis: suitable

UniProt accession no.

foreign activity

ATPase Activity ≤0.01 units/mg protein

storage temp.

−20°C

Gene Information

General description

Myosin heavy chain (MHC) is a motor protein of muscle thick filaments. MHC regulates ultrastructural diversity of myofibrils.

Application

Myosin Heavy Chain from rabbit muscle has been used:
  • in SDS analysis of myosin heavy chain (MHC) composition and content
  • for microinjection into the outflow of heart (blood serum) and the cephalic cavities (embryonic cerebrospinal fluid) of chick embryo
  • as a muscle protein, to study the role of ascorbate as a facilitator of glycogen storage in muscles

Biochem/physiol Actions

Molecular motor involved in the contraction of muscles.

Physical form

Solution in 50% glycerol containing 0.6 M KCl and 0.005 M potassium phosphate, pH 6.5

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M1636T2400M4824
biological source

rabbit muscle

biological source

rabbit muscle

biological source

Porcine muscle

biological source

human heart

technique(s)

electrophoresis: suitable

technique(s)

ELISA: suitable

technique(s)

electrophoresis: suitable

technique(s)

-

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

lyophilized powder (contains Tris buffer salts)

form

lyophilized

UniProt accession no.

Q07003

UniProt accession no.

-

UniProt accession no.

-

UniProt accession no.

P05976, P08590, P10916

Gene Information

rabbit ... PBV1SPCR2(100009284)

Gene Information

-

Gene Information

-

Gene Information

human ... MYL1(4632), MYL2(4633), MYL3(4634)

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C


Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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Questions

  1. Does M7659 product Myosin Heavy Chain from rabbit muscle consist of only one myosin heavy chain isoform or is it a mixture of isoforms? If it is a mixture, could you please provide additional information about its composition?

    1 answer
    1. This product is a mixture of heavy chain isoforms. Further identification of each has not been performed, however on electrophoresis gels, there is a primary band at approximately 200 kDa with a more faint, slightly lower molecular weight band present. This may indicate a higher concentration of MyHC I, however this has not been verified.

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