M4937

Anti-Melanocortin-3 Receptor antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(s): Anti-MC3R
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD03455428
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ARR, WB
• Citations: 8

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 40 kDa
• species reactivity: rat, mouse (predicted)
• technique(s): microarray: suitable, western blot: 1:2,000 using rat brain homogenate extract
• UniProt accession no.: P32244
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: mouse ... Mc3r(17201) ; rat ... Mc3r(29310)

Description

General description

Melanocortins are regulatory peptides derived from post-translational processing of the larger pro-opiomelanocortin (POMC) precursor. Melanocortin-3 Receptor (MC3-R) is a 360 amino acid containing transmembrane protein that belongs to G-protein coupled receptor family and is expressed mainly in adult central nervous system at high levels in a restricted number of neurons of the hypothalamus and the limbic system.

Immunogen

A synthetic peptide corresponding to the N-terminal region of rat melanocortin receptor 3 (amino acids 15-33) coupled to keyhole limpet hemocyanin (KLH). This sequence is highly conserved in mouse MC3-R (89% identity).

Application

Anti-Melanocortin-3 Receptor antibody produced in rabbit has been used in western blotting and immunocytochemistry.

Biochem/physiol Actions

Melanocortin-3 (MC3) receptor has a crucial role in facilitating the effects of the melanocortin system on energy homeostasis. The MC3 receptor has been proposed to mediate the actions of MSH, in particular those of α-MSH and γ-MSH peptides. MC3-R is thought to regulate fat stores by a metabolic pathway. MC3-R knockout mice (MC3-R-/-) have increased fat mass, but not high body mass index, are hyperleptinaemic and develop mild hyperinsulinaemia. In contrast to MC3-R, MC4-R primarily controls food intake, suggesting that both MC3 and MC4 receptors may play a complementary role in weight control.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable