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L8647

Sigma-Aldrich

Levan from Erwinia herbicola

≥98% (TLC)

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352201
NACRES:
NA.25

biological source

bacterial (Erwinia herbicola)

Quality Level

Assay

≥98% (TLC)

form

powder

technique(s)

thin layer chromatography (TLC): suitable

color

white

solubility

water: 9.80-10.20 mg/mL, cloudy to hazy, colorless to faintly yellow

storage temp.

2-8°C

InChI

1S/C18H32O16/c19-1-7-10(23)14(27)17(5-21,33-7)31-3-9-12(25)15(28)18(6-22,34-9)30-2-8-11(24)13(26)16(29,4-20)32-8/h7-15,19-29H,1-6H2/t7-,8-,9-,10-,11-,12-,13+,14+,15+,16-,17-,18-/m1/s1

InChI key

ZFTFOHBYVDOAMH-XNOIKFDKSA-N

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General description

Levan is a polysaccharide produced by some microorganisms and plants. It is a non-typical biopolymer composed of fructose units linked by β-(2→6). Levan is an exopolysaccharide (synthesized outside the cell).

Application

Levan from Erwinia herbicola has been used as a:
  • standard for the nuclear magnetic resonance (NMR) spectroscopy analysis of levan produced by Bacillus licheniformis NS032 in sugar beet molasses-based medium
  • polysaccharide substrate to test the ability of Prevotella copri isolates to grow when polysaccharides were provided as the sole carbon source in vitro
  • substrate in levanase activity assay

Biochem/physiol Actions

Levan has various industrial applications like cosmetics, medicine, or nanotechnology. It might exhibit anti-hyperglycemic effect and anti-tumor action, prevent diabetes-induced oxidative stress and increase catalase (CAT) and superoxide dismutase (SOD) in the heart. Levan is also recognized for its immunomodulating and prebiotic actions.

Other Notes

A polymer of β-D-fructose
To gain a comprehensive understanding of our extensive range of Polysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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G D Gojgic-Cvijovic et al.
International journal of biological macromolecules, 121, 142-151 (2018-10-07)
The production of levan by Bacillus licheniformis NS032 in a medium based on sugar beet molasses was studied. High polysaccharide yields were produced by using diluted molasses (100-140 g/L of total sugars) with the addition of commercial sucrose up to 200 g/L
Payal Joglekar et al.
mSphere, 3(3) (2018-05-26)
Genomic differences between gut-resident bacterial strains likely underlie significant interindividual variation in microbiome function. Traditional methods of determining community composition, such as 16S rRNA gene amplicon sequencing, fail to capture this functional diversity. Metagenomic approaches are a significant step forward
Susanne L Jensen et al.
International journal of biological macromolecules, 85, 514-521 (2016-01-17)
A Levanase, LevB, from Bacillus subtilis 168, was expressed as a His6-tagged protein in Escherichia coli. The enzyme was purified and characterised for its activity and substrate specificity. LevB has a pH optimum of 6.0-6.5 and a maximum observed specific
An evaluation of the prebiotic potential of microbial levans from Erwinia sp. 10119
Liu C, et al.
Journal of functional foods, 64, 103668-103668 (2020)
Hannah Fehlner-Peach et al.
Cell host & microbe, 26(5), 680-690 (2019-11-15)
Gut-dwelling Prevotella copri (P. copri), the most prevalent Prevotella species in the human gut, have been associated with diet and disease. However, our understanding of their diversity and function remains rudimentary because studies have been limited to 16S and metagenomic surveys

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