L5520
L-15 Medium (Leibovitz)
Without L-glutamine, liquid, sterile-filtered, suitable for cell culture
Synonym(s):
Leibovitz L15
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Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
phenol red: 0.011 g/L
sodium pyruvate: 0.55 g/L
shipped in
ambient
storage temp.
2-8°C
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General description
L-15 Medium (Leibovitz) was initially prepared to be used in carbon dioxide (CO2) free systems, which involves sodium bicarbonate. The complement of free base amino acids, salts, and galactose buffers it. This aids the maintenance of physiological pH control.[1]
Application
L-15 Medium (Leibovitz) has been used:
- in protein incorporation for incubation of high blastula embryos and to avoid the abnormal development of embryos[2]
- as a supplement in sterile cell media for the dissection and isolation of kidney cells[3]
- as a component of culture buffer for the tissue culture of Newt lung cells[4]
Reconstitution
Supplement with 0.3 g/L L-glutamine.
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supplement
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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